Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Physiological evidence for the presence of a cis-trans isomerase of unsaturated fatty acids in Methylococcus capsulatus Bath to adapt to the presence of toxic organic compounds
Autor Löffler, C.; Eberlein, C.; Mäusezahl, I.; Kappelmeyer, U.; Heipieper, H.J.;
Journal / Serie FEMS Microbiology Letters
Erscheinungsjahr 2010
Department UBT;
Band/Volume 308
Heft 1
Sprache englisch;
Keywords Methylococcus capsulatus Bath; solvent stress; adaptation; cis-trans isomerase
Abstract The physiology of the response in the methanotrophic bacterium Methylococcus capsulatus Bath towards thermal and solvent stress was studied. A systematic investigation of the toxic effects of organic compounds (chlorinated phenols and alkanols) on the growth of this bacterium was carried out. The sensitivity to the tested alkanols correlated with their chain length and hydrophobicity; methanol was shown to be an exception to which the cells showed a very high tolerance. This can be explained by the adaptation of these bacteria to growth on C1 compounds. On the other hand, M. capsulatus Bath was very sensitive towards the tested chlorinated phenols. The high toxic effect of phenolic compounds on methanotrophic bacteria might be explained by the occurrence of toxic reactive oxygen species. In addition, a physiological proof of the presence of cis-trans isomerization as a membrane-adaptive response mechanism in M. capsulatus was provided. This is the first report on physiological evidence for the presence of the unique postsynthetic membrane-adaptive response mechanism of the cis-trans isomerization of unsaturated fatty acids in a bacterium that does not belong to the genera Pseudomonas and Vibrio where this mechanism was already reported and described extensively.
ID 10266
dauerhafte UFZ-Verlinkung http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10266
Löffler, C., Eberlein, C., Mäusezahl, I., Kappelmeyer, U., Heipieper, H.J. (2010):
Physiological evidence for the presence of a cis-trans isomerase of unsaturated fatty acids in Methylococcus capsulatus Bath to adapt to the presence of toxic organic compounds
FEMS Microbiol. Lett. 308 (1), 68 - 75