Details zur Publikation

Referenztyp Buchkapitel
DOI / URL Link
Titel (primär) Assessing the mycorrhizal diversity of soils and identification of fungus fruiting bodies and axenic cultures
Titel (sekundär) Symbiotic fungi. Principles and practice
Autor Krüger, D.; Sharma, M.; Varma, A.;
Herausgeber Varma, A.; Kharkwal, A.C.;
Journal / Serie Soil Biology
Erscheinungsjahr 2009
Department BOOEK;
Band/Volume 18
Sprache englisch;
UFZ Bestand Halle - Bibliothek, 09-1011, 00354554
Abstract For assessment of mycorrhizal diversity of soils and for identification of fungal fruiting bodies, many techniques are used. The classical fungal identification starts with collection of field notes, and laboratory processing which involves the isolation of fungal cultures from soils, fruiting bodies, and spores by using direct or indirect plating methods for microbial screening. The wet-sieving and decanting technique for the extraction of spores of arbuscular mycorrhizal fungi (AMF) and microscopic identification are other important techniques. While the work with specimens and cultures is described, it is worthy of note that direct application of molecular methods to environmental material can detect many more. Yet often different sets of fungal taxa are involved, as for example shown for the large basidiomycete diversity in agricultural soil. The advances and cost reduction in DNA-based identification of biological material has greatly improved the catalog of methods available to soil ecologists. The modern molecular method used in fungal identification of a fungal specimen or culture is to make nucleic acid available for amplification, done by the polymerase chain reaction (PCR). Commercialized and home-made "extraction-free" methods for many biological organisms are available; a simple method is PEX extraction. It involves the unique activity of liquid polyethylene glycol 200 (PEG200) in cell disruption and DNA stabilization. Other tools such as sequencing and editing sequences, cloning, database queries and alignment, where one can BLAST multiple sequences together and can also align query sequences with similar sequences, are available. Phylogenetic placement, a phenetics-based distance method, is also used to simplify fungal identification.
ID 10212
dauerhafte UFZ-Verlinkung
Krüger, D., Sharma, M., Varma, A. (2009):
Assessing the mycorrhizal diversity of soils and identification of fungus fruiting bodies and axenic cultures
In: Varma, A., Kharkwal, A.C. (eds.)
Symbiotic fungi. Principles and practice
Soil Biology 18
Springer, Berlin, Heidelberg, p. 159 - 188