Details zur Publikation

Referenztyp Buchkapitel
DOI / URL Link
Titel (primär) In situ microcosms studies to characterize microbial processes in the field
Titel (sekundär) Experimental protocols and appendices
Autor Kästner, M.; Richnow, H.H.;
Herausgeber Timmis, K.N.; McGenity, T.; van den Meer, J.R.; de Lorenzo, V.;
Journal / Serie Handbook of Hydrocarbon and Lipid Microbiology
Erscheinungsjahr 2010
Department ISOBIO; UBT;
Band/Volume Vol. 5, Part 1
Sprache englisch;
UFZ Bestand Leipzig, Bibliothek, Hauptlesesaal, 00358415, 09-1344 DK: 579.222.5 Han
Abstract A concept of in situ microcosms, which allows analyzing structure, function, and activity of microbial communities in situ, as well as the enrichment of microorganisms for subsequent cultivation in laboratory experiments is described. The concept is based on the application of stable isotope-labeled compounds supplied within the microcosm system. Microorganisms capable of utilizing these substrates as carbon or nitrogen sources become labeled upon metabolism and biosynthesis. Stable isotope probing (SIP) of biomarker molecules such as?fatty acids, proteins, or nucleic acids allows tracing the flux of the label into the biomass and?offers potential for linking structure and function of the active microbial communities.
ID 10146
dauerhafte UFZ-Verlinkung http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10146
Kästner, M., Richnow, H.H. (2010):
In situ microcosms studies to characterize microbial processes in the field
In: Timmis, K.N., McGenity, T., van den Meer, J.R., de Lorenzo, V. (eds.)
Experimental protocols and appendices
Handbook of Hydrocarbon and Lipid Microbiology Vol. 5, Part 1
Springer International Publishing, Cham, p. 3504 - 3511