Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Autoproteolytic stability of a trypsin from the marine crab Cancer pagurus
Autor Hehemann, J.H.; Redecke, L.; Murugaiyan, J.; von Bergen, M.; Betzel, C.; Saborowski, R.;
Journal / Serie Biochemical and Biophysical Research Communications
Erscheinungsjahr 2008
Department PROTEOM;
Band/Volume 370
Heft 4
Sprache englisch;
Keywords Crustacea; Crab; Cancer pagurus; Gastric fluid; Digestive enzymes; Trypsin; Autoproteolysis; Stability
Abstract Autoproteolytic stability is a crucial factor for the application of proteases in biotechnology. In contrast to vertebrate enzymes, trypsins from shrimp and crayfish are known to be resistant against autolysis. We show by characterisation of a novel trypsin from the gastric fluid of the marine crab Cancer pagurus that this property might be assigned to the entire class of crustaceans. The isolated and cloned crab trypsin (C.p.TryIII) exhibits all characteristic properties of crustacean trypsins. However, its overall sequence identity to other trypsins of this systematic class is comparatively low. The high resistance against autoproteolysis was determined by mass spectrometry, which revealed a low susceptibility of the N-terminal domain towards autolysis. By homology modelling of the tertiary structure, the elevated stability was attributed to the distinctly different pattern of autolytic cleavage sites, which is conserved in all known crustacean trypsin sequences.
ID 1014
dauerhafte UFZ-Verlinkung
Hehemann, J.H., Redecke, L., Murugaiyan, J., von Bergen, M., Betzel, C., Saborowski, R. (2008):
Autoproteolytic stability of a trypsin from the marine crab Cancer pagurus
Biochem. Biophys. Res. Commun. 370 (4), 566 - 571