Details zur Publikation

Referenztyp Buchkapitel
DOI / URL Link
Titel (primär) Cis-trans isomerase of unsaturated fatty acids: an immediate bacterial adaptive mechanism to cope with emerging membrane perturbation caused by toxic hydrocarbons
Titel (sekundär) Microbial utilization of hydrocarbons, oils and lipids
Autor Heipieper, H.J.; Fischer, J.; Meinhardt, F.;
Herausgeber Timmis, K.N.; McGenity, T.; van den Meer, J.R.; de Lorenzo, V.;
Journal / Serie Handbook of Hydrocarbon and Lipid Microbiology
Erscheinungsjahr 2010
Department UBT;
Band/Volume Vol. 2, Part 9
Sprache englisch;
UFZ Bestand Leipzig, Bibliothek, Hauptlesesaal, 00358095, 09-1341 DK: 579.222.5 Han
Abstract A rather efficient solvent adaptation mechanism enabling several gram-negative bacteria to tolerate and grow in the presence of membrane-disturbing compounds is the isomerization of cis- to trans-unsaturated membrane fatty acids. The degree of isomerization obviously depends on the toxicity and the concentration of membrane-affecting agents. Synthesis of trans-fatty acids comes about by direct isomerization of the respective cis-configuration of the double bond without shifting the position. The purpose of the conversion of the cis-configuration to trans is apparently the rapid adaptation of the membrane fluidity to rising temperature or the presence of toxic organic hydrocarbons. The cis-trans-isomerase (Cti) is a constitutively expressed periplasmic enzyme that - to exert its action - necessitates neither ATP nor other cofactors, and consistently, is independent of de novo synthesis of lipids. A heme-binding site typical of cytochrome c-type proteins is present in the predicted Cti polypeptide indicating a reaction mechanism that renounces temporary saturation of the double bond. Due to its direct correlation with toxicity, cis-trans-isomerization is a potential biomarker for recording solvent stress or changes of other environmental conditions.
ID 10070
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10070
Heipieper, H.J., Fischer, J., Meinhardt, F. (2010):
Cis-trans isomerase of unsaturated fatty acids: an immediate bacterial adaptive mechanism to cope with emerging membrane perturbation caused by toxic hydrocarbons
In: Timmis, K.N., McGenity, T., van den Meer, J.R., de Lorenzo, V. (eds.)
Microbial utilization of hydrocarbons, oils and lipids
Handbook of Hydrocarbon and Lipid Microbiology Vol. 2, Part 9
Springer International Publishing, Cham, p. 1605 - 1614