Isotope Lab

Equipment

The Laboratory of Stable Isotopes (LSI) is fully equipped for bulk stable isotope analysis and compound specific isotope analysis of organic compounds (2H, 13C, 15N, 18O), providing solutions for a board spectrum of analytical problems.
Organic chemical analyses by gas chromatography with mass selective detection (GC-MS) and flame ionization detection (GC-FID) is performed to identify and monitor characteristic compounds.

1 MAT 252 with GC/C III interface - 13C, 15N
2 MAT 253 with GC-IsoLink - 2H, 13C, 15N
1 MAT 253 with GC-IsoLink - 2H, 13C, 15N; EA - 13C, 15N; Pyrolysis - 2H, 18O
1 MAT 253 with GC-IsoLink - 2H, 13C, 15N; HPLC - 13C
1 GC-MS
3 GC-FID
1 HPLC


Elemental Analyser (EA) – 13C, 15N
Solid and liquid samples are flash combusted in a helium gas flow in the EA. The produced gases of CO2 and N2 are transported via open split (ConFlo IV) in the IRMS and the isotope ratios of 13C and 15N are determined.

High Temperature Pyrolysis (HTP) – 18O, 2H
Solid and liquid samples are thermally chemical decomposed (Temperature 1350-1500 °C) in a helium gas flow in the HTP. The produced gasses of CO and H2 are transported via open split (ConFlo IV) in the IRMS and the isotope ratios of 18O and 2H are determined.

GC-Combustion (GC/C) – 13C, 15N
Organic mixtures are separated by a gas chromatopgraphy system. The separated individual compounds are online combusted (940-1000 °C) and the generated gases of CO2 or N2 are transported via open split (ConFlo IV) into the IRMS and the isotope ratios of 13C and 15N are determined.

GC-High Temperature Conversion (GC/HTC) – 2H
Organic mixtures are separated by a gas chromatography system. The separated individual compounds are online decomposed (1450 °C) to produce H2 which is isotopically characterized by IRMS.

HPLC-Liquid Chemical Oxidation (compound specific and µ-bulk) – 13C
Compounds are separated in aqueous phase using inorganic buffers. The LC IsoLink allows quantitative, fractionation-free elimination of the liquid phase prior to the oxidative conversion of the sample into CO2 and after measurement of 13C isotope ratio of each separated component.

Isotope Lab