Dr. Ivonne Nijenhuis

Contact / Address:

E-mail

Phone + 49 (0) 341 235 1356
Fax +49 (0) 341 235 450822


Helmholtz Centre for Environmental Research - UFZ
Permoserstrasse 15
04318 Leipzig

Dr. Ivonne Nijenhuis

Curriculum Vitae


2004 -
current
Senior Scientist, Department Isotope Biogeochemistry
2010-2015 Coordinator European Union FP7 'People' programme Initial Training Network CSI:ENVIRONMENT
2002 -
2004
Post-Doctoral Scientist EU Marie Curie Host Development fellowship (BIOISOTOPE) European expert in Anaerobic Microbiology, UFZ-Leipzig
2002 Ph.D. in Microbiology; Cornell University, USA
1996 M.Sc. in Environmental Sciences; Wageningen Agricultural University, the Netherlands

Research areas

My research in the Department of Isotope Biogeochemistry focuses on the anaerobic biotransformation of halogenated organic contaminants and the application of stable isotope concepts. Currently, biotransformation of the chlorinated and brominated ethenes and ethanes but also halogenated pesticides, e.g. hexachlorocyclohexane, and pharmaceuticals, e.g. diclofenac are investigated at different scales, from laboratory enzyme assays and cultures to model scale wetland and in situ at contaminated field sites. My research involves enrichment and isolation approaches for investigation of novel microorganisms capable of biotransformation of the halogenated organic contaminants as well as the development and application of compound-specific stable isotope (C, Cl, Br, H) fractionation of these contaminants as a tool to analyse reactions and biotransformation in situ.


Current projects

Early 2011 the DFG Forschergruppe "FOR1530 'Anaerobic Dehalogenation: Organisms, Biochemistry and (Eco-) Physiology'" started linking research groups in Jena, Halle and Leipzig. Within this project, I am in charge of sub-project 05: 'Characterisation of microbial dehalogenation using compound specific stable isotope analysis'.


Completed projects


Within the European Union FP7 project "GENESIS" we investigated how biogeochemical processes affect natural attenuation of chlorinated benzenes and are focusing on the microbial community involved in contaminant biotransformation.

From 2010-2015, I am coordinated the European Union FP7 'People' programme Initial Training Network "CSI:ENVIRONMENT". In this project, 10 Partners and 4 Associated Partners from 9 European countries will work on 'Isotope forensics meets biogeochemistry - linking sources and sinks of organic contaminants by compound specific isotope investigation'.

2012-2014, Joint German-Israeli Water Technology Programme “Development of innovative tools for the assessment of in situ biodegradation of organobromine contaminant in groundwater” – INTIME. In this 3 year project we will investigate the application of CSIA for assessment of the natural attenuation of brominated groundwater contaminants.


Publications (in co-operation with the UFZ)

  • Bashir, S., Fischer, A., Nijenhuis, I., Richnow, H.H., (2013):
    Enantioselective carbon stable isotope fractionation of hexachlorocyclohexane during aerobic biodegradation by Sphingobium spp.
    Environ. Sci. Technol. 47 (20), 11432 - 11439
    full text (url)
  • Birkigt, J., Gilevska, T., Ricken, B., Richnow, H.-H., Vione, D., Corvini, P.F.-X., Nijenhuis, I., Cichocka, D., (2015):
    Carbon stable isotope fractionation of sulfamethoxazole during biodegradation by Microbacterium sp. strain BR1 and upon direct photolysis
    Environ. Sci. Technol. 49 (10), 6029 - 6036
    full text (url)
  • Cichocka, D., Imfeld, G., Richnow, H.H., Nijenhuis, I., (2008):
    Variability in microbial carbon isotope fractionation of tetra- and trichloroethene upon reductive dechlorination
    Chemosphere 71 (4), 639 - 648
    full text (url)
  • Cichocka, D., Nikolausz, M., Haest, P.J., Nijenhuis, I., (2010):
    Tetrachloroethene conversion to ethene by a Dehalococcoides-containing enrichment culture from Bitterfeld
    FEMS Microbiol. Ecol. 72 (2), 297 - 310
    full text (url)
  • Cichocka, D., Siegert, M., Imfeld, G., Andert, J., Beck, K., Diekert, G., Richnow, H.H., Nijenhuis, I., (2007):
    Factors controlling the carbon isotope fractionation of tetra- and trichloroethene during reductive dechlorination by Sulfurospirillum ssp. and Desulfitobacterium sp. strain PCE-S
    FEMS Microbiol. Ecol. 62 (1), 98 - 107
    full text (url)
  • Duldhardt, I., Gaebel, J., Chrzanowski, Ł., Nijenhuis, I., Schauer, F., Heipieper, H.J., (2010):
    Adaptation of anaerobically grown Thauera aromatica, Geobacter sulfurreducens and Desulfococcus multivorans to organic solvents on the level of membrane fatty acid composition
    Microb. Biotechnol. 3 (2), 201 - 209
    full text (url)
  • Duldhardt, I., Nijenhuis, I., Schauer, F., Heipieper, H.J., (2007):
    Anaerobically grown Thauera aromatica, Desulfococcus multivorans, Geobacter sulfurreducens are more sensitive towards organic solvents than aerobic bacteria
    Appl. Microbiol. Biotechnol. 77 (3), 705 - 711
    full text (url)
  • Elsayed, O.F., Maillard, E., Vuilleumier, S., Nijenhuis, I., Richnow, H.H., Imfeld, G., (2014):
    Using compound-specific isotope analysis to assess the degradation of chloroacetanilide herbicides in lab-scale wetlands
    Chemosphere 99 , 89 - 95
    full text (url)
  • Ewald, E.-M., Wagner, A., Nijenhuis, I., Richnow, H.H., Lechner, U., (2007):
    Microbial dehalogenation of trichlorinated dibenzo-p-dioxins by a Dehalococcoides-containing mixed culture is coupled to carbon isotope fractionation
    Environ. Sci. Technol. 41 (22), 7744 - 7751
    full text (url)
  • Fennell, D.E., Nijenhuis, I., Wilson, S.F., Zinder, S.H., Häggblom, M.M., (2004):
    Dehalococcoides ethenogenes strain 195 reductively dechlorinates diverse chlorinated aromatic pollutants
    Environ. Sci. Technol. 38 (7), 2075 - 2081
    full text (url)
  • Filippini, M., Stumpp, C., Nijenhuis, I., Richnow, H.H., Gargini, A., (2015):
    Evaluation of aquifer recharge and vulnerability in an alluvial lowland using environmental tracers
    J. Hydrol. 529 , 1657 - 1668
    full text (url)
  • Fletcher, K.E., Löffler, F.E., Richnow, H.-H., Nijenhuis, I., (2009):
    Stable carbon isotope fractionation of 1,2-Dichloropropane during dichloroelimination by Dehalococcoides populations
    Environ. Sci. Technol. 43 (18), 6915 - 6919
    full text (url)
  • Fletcher, K.E., Nijenhuis, I., Richnow, H.-H., Löffler, F.E., (2011):
    Stable carbon isotope enrichment factors for cis-1,2-dichloroethene and vinyl chloride reductive dechlorination by Dehalococcoides
    Environ. Sci. Technol. 45 (7), 2951 - 2957
    full text (url)
  • Herrero-Martín, S., Nijenhuis, I., Richnow, H.H., Gehre, M., (2015):
    Coupling of a headspace autosampler with a programmed temperature vaporizer for stable carbon and hydrogen isotope analysis of volatile organic compounds at microgram per liter concentrations
    Anal. Chem. 87 (2), 951 - 959
    full text (url)
  • Imfeld, G., Aragonés, C.E., Fetzer, I., Mészáros, E., Zeiger, S., Nijenhuis, I., Nikolausz, M., Delerce, S., Richnow, H.H., (2010):
    Characterization of microbial communities in the aqueous phase of a constructed model wetland treating 1,2-dichloroethene-contaminated groundwater
    FEMS Microbiol. Ecol. 72 (1), 74 - 88
    full text (url)
  • Imfeld, G., Nijenhuis, I., Nikolausz, M., Zeiger, S., Paschke, H., Drangmeister, J., Grossmann, J., Richnow, H.H., Weber, S., (2008):
    Assessment of in situ degradation of chlorinated ethenes and bacterial community structure in a complex contaminated groundwater system
    Water Res. 42 (4-5), 871 - 882
    full text (url)
  • Imfeld, G., Pieper, H., Shani, N., Rossi, P., Nikolausz, M., Nijenhuis, I., Paschke, H., Weiß, H., Richnow, H.H., (2011):
    Characterization of groundwater microbial communities, dechlorinating bacteria, and in situ biodegradation of chloroethenes along a vertical gradient
    Water Air Soil Pollut. 221 (1-4), 107 - 122
    full text (url)
  • Kaschl, A., Vogt, C., Uhlig, S., Nijenhuis, I., Weiß, H., Kästner, M., Richnow, H.H., (2005):
    Isotopic fractionation indicates anaerobic monochlorobenzene biodegradation
    Environ. Toxicol. Chem. 24 (6), 1315 - 1324
    full text (url)
  • Kästner, M., Fischer, A., Nijenhuis, I., Geyer, R., Stelzer, N., Bombach, P., Tebbe, C.C., Richnow, H.H., (2006):
    Assessment of microbial in situ activity in contaminated aquifers
    Eng. Life Sci. 6 (3), 234 - 251
    full text (url)
  • Kaufhold, T., Schmidt, M., Cichocka, D., Nikolausz, M., Nijenhuis, I., (2013):
    Dehalogenation of diverse halogenated substrates by a highly enriched Dehalococcoides-containing culture derived from the contaminated mega-site in Bitterfeld
    FEMS Microbiol. Ecol. 83 (1), 176 - 188
    full text (url)
  • Knight, V.K., Nijenhuis, I., Kerkhof, L.J., Häggblom, M.M., (2002):
    Degradation of aromatic compounds coupled to selenate reduction
    Geomicrobiol. J. 19 (1), 77 - 86
    full text (url)
  • Kret, E., Kiecak, A., Malina, G., Nijenhuis, I., Postawa, A., (2015):
    Identification of TCE and PCE sorption and biodegradation parameters in a sandy aquifer for fate and transport modelling: batch and column studies
    Environ. Sci. Pollut. Res. 22 (13), 9877 - 9888
    full text (url)
  • Langenhoff, A., Nijenhuis, I., Tan, N.C.G., Briglia, M., Zehnder, A.J.B., Schraa, G., (1997):
    Characterisation of a manganese-reducing, toluene-degrading enrichment culture
    FEMS Microbiol. Ecol. 24 (2), 113 - 125
    full text (url)
  • Liu, F., Cichocka, D., Nijenhuis, I., Richnow, H.H., Fennell, D.E., (2010):
    Carbon isotope fractionation during dechlorination of 1,2,3,4-tetrachlorodibenzo-p-dioxin by a Dehalococcoides-containing culture
    Chemosphere 80 (10), 1113 - 1119
    full text (url)
  • Lünsmann, V., Kappelmeyer, U., Taubert, A., Nijenhuis, I., von Bergen, M., Heipieper, H.J., Müller, J.A., Jehmlich, N., (2016):
    Aerobic toluene degraders in the rhizosphere of a constructed wetland model show diurnal polyhydroxyalkanoate metabolism
    Appl. Environ. Microb.
    full text (url)
  • Marco-Urrea, E., Nijenhuis, I., Adrian, L., (2011):
    Transformation and carbon isotope fractionation of tetra- and trichloroethene to Trans-Dichloroethene by Dehalococcoides sp. strain CBDB1
    Environ. Sci. Technol. 45 (4), 1555 - 1562
    full text (url)
  • Martínez-Lavanchy, P.M., Chen, Z., Lünsmann, V., Marin-Cevada, V., Vilchez-Vargas, R., Pieper, D.H., Reiche, N., Kappelmeyer, U., Imparato, V., Junca, H., Nijenhuis, I., Müller, J.A., Kuschk, P., Heipieper, H.J., (2015):
    Microbial toluene removal in hypoxic model constructed wetlands occurs predominantly via the ring monooxygenation pathway
    Appl. Environ. Microb. 81 (18), 6241 - 6252
    full text (url)
  • Martínez-Lavanchy, P.M., Dohrmann, A.B., Imfeld, G., Trescher, K., Tebbe, C.C., Richnow, H.-H., Nijenhuis, I., (2011):
    Detection of monochlorobenzene metabolizing bacteria under anoxic conditions by DNA-stable isotope probing
    Biodegradation 22 (5), 973 - 982
    full text (url)
  • Martínez-Lavanchy, P.M., Müller, C., Nijenhuis, I., Kappelmeyer, U., Buffing, M., McPherson, K., Heipieper, H.J., (2010):
    High stability and fast recovery of expression of the TOL plasmid-carried toluene catabolism genes of Pseudomonas putida mt-2 under conditions of oxygen limitation and oscillation
    Appl. Environ. Microb. 76 (20), 6715 - 6723
    full text (url)
  • Maymó-Gatell, X., Nijenhuis, I., Zinder, S.H., (2001):
    Reductive dechlorination of cis-1,2-dichlorethene and vinyl chloride by "Dehalococcoides ethenogenes"
    Environ. Sci. Technol. 35 (3), 516 - 521
    full text (url)
  • Mészáros, E., Imfeld, G., Nikolausz, M., Nijenhuis, I., (2013):
    Occurrence of Dehalococcoides and reductive dehalogenase genes in microcosms, a constructed wetland and groundwater from a chlorinated ethene contaminated field site as indicators for in situ reductive dehalogenation
    Water Air Soil Pollut. 224 (11), 1768
    full text (url)
  • Nijenhuis, I., Andert, J., Beck, K., Kästner, M., Diekert, G., Richnow, H.H., (2005):
    Stable isotope fractionation of tetrachloroethene during reductive dechlorination by Sulfurospirillum multivorans and Desulfitobacterium sp. strain PCE-S and abiotic reactions with cyanocobalamin
    Appl. Environ. Microb. 71 (7), 3413 - 3419
    full text (url)
  • Nijenhuis, I., Kuntze, K., (2016):
    Anaerobic microbial dehalogenation of organohalides — state of the art and remediation strategies
    Curr. Opin. Biotechnol. 38 , 33 - 38
    full text (url)
  • Nijenhuis, I., Martínez, P., Junca, H., Stelzer, N., Imfeld, G., Weber, S., Richnow, H., (2009):
    In situ investigation of microbial diversity and anaerobic chlorobenzene degradation along a vertical gradient
    Geochim. Cosmochim. Acta 73 (13 Suppl.), A943
    full text (url)
  • Nijenhuis, I., Nikolausz, M., Köth, A., Felföldi, T., Weiß, H., Drangmeister, J., Großmann, J., Kästner, M., Richnow, H.H., (2007):
    Assessment of the natural attenuation of chlorinated ethenes in an anaerobic contaminated aquifer in the Bitterfeld/Wolfen area using stable isotope techniques, microcosm studies and molecular biomarkers
    Chemosphere 67 (2), 300 - 311
    full text (url)
  • Nijenhuis, I., Renpenning, J., Kümmel, S., Richnow, H.H., Gehre, M., (2016):
    Recent advances in multi-element compound-specific stable isotope analysis of organohalides: Achievements, challenges and prospects for assessing environmental sources and transformation
    Trends in Environmental Analytical Chemistry 11 , 1 - 8
    full text (url)
  • Nijenhuis, I., Schmidt, M., Pellegatti, E., Paramatti, E., Richnow, H.-H., Gargini, A., (2013):
    A stable isotope approach for source apportionment of chlorinated ethene plumes at a complex multi-contamination events urban site
    J. Contam. Hydrol. 153 , 92 - 105
    full text (url)
  • Nijenhuis, I., Stelzer, N., Kästner, M., Richnow, H.-H., (2007):
    Sensitive detection of anaerobic monochlorobenzene degradation using stable isotope tracers
    Environ. Sci. Technol. 41 (11), 3836 - 3842
    full text (url)
  • Nijenhuis, I., Zinder, S.H., (2005):
    Characterization of hydrogenase and reductive dehalogenase activities of Dehalococcoides ethenogenes strain 195
    Appl. Environ. Microb. 71 (3), 1664 - 1667
    full text (url)
  • Nikolausz, M., Kappelmeyer, U., Nijenhuis, I., Ziller, K., Kästner, M., (2005):
    Molecular characterization of dichloromethane-degrading Hyphomicrobium strains using 16S rDNA and DCM dehalogenase gene sequences
    Syst. Appl. Microbiol. 28 (7), 582 - 587
    full text (url)
  • Nikolausz, M., Nijenhuis, I., Ziller, K., Richnow, H.H., Kästner, M., (2006):
    Stable carbon isotope fractionation during degradation of dichloromethane by methylotrophic bacteria
    Environ. Microbiol. 8 (1), 156 - 164
    full text (url)
  • Pöritz, M., Goris, T., Wubet, T., Tarkka, M.T., Buscot, F., Nijenhuis, I., Lechner, U., Adrian, L., (2013):
    Genome sequences of two dehalogenation specialists - Dehalococcoides mccartyi strains BTF08 and DCMB5 enriched from the highly polluted Bitterfeld region
    FEMS Microbiol. Lett. 343 (2), 101 - 104
    full text (url)
  • Pöritz, M., Schiffmann, C.L., Hause, G., Heinemann, U., Seifert, J., Jehmlich, N., von Bergen, M., Nijenhuis, I., Lechner, U., (2015):
    Dehalococcoides mccartyi strain DCMB5 respires a broad spectrum of chlorinated aromatic compounds
    Appl. Environ. Microb. 81 (2), 587 - 596
    full text (url)
  • Renpenning, J., Hitzfeld, K.L., Gilevska, T., Nijenhuis, I., Gehre, M., Richnow, H.-H., (2015):
    Development and validation of an universal interface for compound-specific stable isotope analysis of chlorine (37Cl/35Cl) by GC-high-temperature conversion (HTC)-MS/IRMS
    Anal. Chem. 87 (5), 2832 - 2839
    full text (url)
  • Renpenning, J., Keller, S., Cretnik, S., Shouakar-Stash, O., Elsner, M., Schubert, T., Nijenhuis, I., (2014):
    Combined C and Cl isotope effects indicate differences between corrinoids and enzyme (Sulfurospirillum multivorans PceA) in reductive dehalogenation of tetrachloroethene, but not trichloroethene
    Environ. Sci. Technol. 48 (20), 11837 - 11845
    full text (url)
  • Renpenning, J., Rapp, I., Nijenhuis, I., (2015):
    Substrate hydrophobicity and cell composition influence the extent of rate limitation and masking of isotope fractionation during microbial reductive dehalogenation of chlorinated ethenes
    Environ. Sci. Technol. 49 (7), 4293 - 4301
    full text (url)
  • Schmidt, M., Lege, S., Nijenhuis, I., (2014):
    Comparison of 1,2-dichloroethane, dichloroethene and vinyl chloride carbon stable isotope fractionation during dechlorination by two Dehalococcoides strains
    Water Res. 52 , 146 - 154
    full text (url)
  • Schmidt, M., Wolfram, D., Birkigt, J., Ahlheim, J., Paschke, H., Richnow, H.-H., Nijenhuis, I., (2014):
    Iron oxides stimulate microbial monochlorobenzene in situ transformation in constructed wetlands and laboratory systems
    Sci. Total Environ. 472 , 185 - 193
    full text (url)
  • Stelzer, N., Büning, C., Pfeifer, F., Dohrmann, A.B., Tebbe, C.C., Nijenhuis, I., Kästner, M., Richnow, H.H., (2006):
    In situ microcosms to evaluate natural attenuation potentials in contaminated aquifers
    Org. Geochem. 37 (10), 1394 - 1410
    full text (url)
  • Stelzer, N., Imfeld, G., Thullner, M., Lehmann, J., Poser, A., Richnow, H.-H., Nijenhuis, I., (2009):
    Integrative approach to delineate natural attenuation of chlorinated benzenes in anoxic aquifers
    Environ. Pollut. 157 (6), 1800 - 1806
    full text (url)
  • Zhang, N., Bashir, S., Qin, J., Schindelka, J., Fischer, A., Nijenhuis, I., Herrmann, H., Wick, L.Y., Richnow, H.H., (2014):
    Compound specific stable isotope analysis (CSIA) to characterize transformation mechanisms of α-hexachlorocyclohexane
    J. Hazard. Mater. 280 , 750 - 757
    full text (url)