Press release from 11th of August 2011
Hydrogen highway in the deep sea
Max Planck Researchers discover hydrogen-powered symbiotic bacteria in deep-sea hydrothermal vent mussels
The search for new energy sources to power mankind’s increasing needs is currently a topic of immense interest. Hydrogen-powered fuel cells are considered one of the most promising clean energy alternatives. While intensive research efforts have gone into developing ways to harness hydrogen energy to fuel our everyday lives, a natural example of a living hydrogen-powered ‘fuel cell’ has gone unnoticed.
During a recent expedition to hydrothermal vents in the deep sea, researchers from the Max Planck Institute of Marine Microbiology and the Cluster of Excellence MARUM discovered mussels that have their own on-board ‘fuel cells’, in the form of symbiotic bacteria that use hydrogen as an energy source. Their results, which appear in the current issue of Nature, suggest that the ability to use hydrogen as a source of energy is widespread in hydrothermal vent symbioses.
Deep-sea hydrothermal vents are formed at mid-ocean spreading centers where tectonic plates drift apart and new oceanic crust is created by magma rising from deep within the Earth. When seawater interacts with hot rock and rising magma, it becomes superheated, dissolving minerals out of the Earth’s crust. At hydrothermal vents, this superheated energy-laden seawater gushes back out into the ocean at temperatures of up to 400 degrees Celsius, forming black smoker chimneys where it comes into contact with cold deep-sea water. These hot fluids deliver inorganic compounds such as hydrogen sulfide, ammonium, methane, iron and hydrogen to the oceans. The organisms living at hydrothermal vents oxidize these inorganic compounds to gain the energy needed to create organic matter from carbon dioxide. Unlike on land, where sunlight provides the energy for photosynthesis, in the dark depths of the sea, inorganic chemicals provide energy for life in a process called chemosynthesis.
When hydrothermal vents were first discovered more than 30 years ago, researchers were astounded to find that they were inhabited by lush communities of animals such as worms, mollusks and crustaceans, most of which were completely unknown to science. The first to investigate these animals quickly realized that the key to their survival was their symbiotic association with chemosynthetic microbes, which are the on-board power plants for hydrothermal vent animals. Until now, only two sources of energy were known to power chemosynthesis by symbiotic bacteria at hydrothermal vents: Hydrogen sulfide, used by sulfur-oxidizing symbionts, and methane, used by methane-oxidizing symbionts. "We have now discovered a third energy source" says Nicole Dubilier from the Max Planck Institute of Marine Microbiology in Bremen, who led the team responsible for this discovery.
The discovery began at the Logatchev hydrothermal vent field, at 3000 m depth on the Mid-Atlantic Ridge, an undersea mountain range halfway between the Caribbean and the Cape Verde Islands. The highest hydrogen concentrations ever measured at hydrothermal vents were recorded during a series of research expeditions to Logatchev. According to Jillian Petersen, a researcher with Nicole Dubilier, "our calculations show that at this hydrothermal vent, hydrogen oxidation could deliver seven times more energy than methane oxidation, and up to 18 times more energy than sulfide oxidation."
In the gills of the deep-sea mussel Bathymodiolus puteoserpentis, one of the most abundant animals at Logatchev, the researchers discovered a sulfur-oxidizing symbiont that can also use hydrogen as an energy source. To track down these hydrogen-powered on-board ‘fuel cells’ in the deep-sea mussels, the researchers deployed two deep-sea submersibles, MARUM-QUEST from MARUM at the University of Bremen, and KIEL 6000 from IFM-GEOMAR in Kiel. With the help of these remotely-driven submersibles, they sampled mussels from sites kilometers below the sea surface. Their ship-board experiments with live samples showed that the mussels consumed hydrogen. Once the samples were back in the laboratory on land, they were able to identify the mussel symbiont hydrogenase, the key enzyme for hydrogen oxidation, using molecular techniques.
The mussel beds at Logatchev form a teeming expanse that covers hundreds of square meteres and contains an estimated half a million mussels. "Our experiments show that this mussel population could consume up to 5000 liters of hydrogen per hour" according to Frank Zielinski, a former doctoral student in Nicole Dubilier’s Group in Bremen, who now works as a post-doctoral researcher at the Helmholtz Centre for Environmental Research in Leipzig. The deep-sea mussel symbionts therefore play a substantial role as the primary producers responsible for transforming geofuels to biomass in these habitats. "The hydrothermal vents along the mid-ocean ridges that emit large amounts of hydrogen can therefore be likened to a hydrogen highway with fuelling stations for symbiotic primary production," says Jillian Petersen.
Even the symbionts of other hydrothermal vent animals such as the giant tubeworm Riftia pachyptila and the shrimp Rimicaris exoculata have the key gene for hydrogen oxidation, but remarkably, this had not been previously recognized. "The ability to use hydrogen as an energy source seems to be widespread in these symbioses, even at hydrothermal vent sites with low amounts of hydrogen," says Nicole Dubilier.
Jillian M. Petersen, Frank U. Zielinski, Thomas Pape, Richard Seifert, Cristina Moraru, Rudolf Amann, Stephane Hourdez, Peter R. Girguis, Scott D. Wankel, Valerie Barbe, Eric Pelletier,
Dennis Fink, Christian Borowski, Wolfgang Bach, Nicole Dubilier (2011):
Hydrogen is an energy source for hydrothermal vent symbioses. Nature 476, 176-180, 11 August 2011. doi: 10.1038/nature10325
This study was supported by the Max Planck Society, the German Research Foundation (Priority program 1144: "From Mantle to Ocean: Energy-, Material- and Life Cycles at Spreading Axes"), and the Cluster of Excellence "The Ocean in the Earth System" at MARUM, Bremen.
Dr. Jillian Petersen
Max Planck Institute of Marine Microbiology
Telephone +49 421 2028 906
Dr. Jillian Petersen
Dr. Frank Zielinski
Helmholtz Centre for Environmental Research(UFZ)
Telephone +49 341 235 1373
Dr. Frank Zielinski
UFZ press office
Telephone +49 341 235 1635
At the Helmholtz Centre for Environmental Research (UFZ), scientists study the causes and repercussions of far-reaching environmental changes. They conduct research into water resources, biodiversity, the consequences of climate change and ways of adaptation, environmental engineering and biotechnology, bioenergy, how chemicals behave in the environment and their impact on human health, modelling, and aspects of the social sciences. The common theme of this diverse research is that it serves the sustainable use of natural resources and helps permanently protect these vital resources in the face of global change. At its sites in Leipzig, Halle and Magdeburg, UFZ currently employs about 1,000 people. It is financed by the German government as well as the governments of Saxony and Saxony-Anhalt.
The Helmholtz Association helps solve major, pressing challenges facing society, science and industry by means of first-rate research in six core fields: Energy, Earth and the Environment, Health, Key Technologies, the Structure of Matter, Transport and Space. With more than 30,000 employees at 17 research centres and an annual budget of some €3 billion, the Helmholtz Association is Germany’s biggest scientific organisation. Its work continues the heritage of scientist Hermann von Helmholtz (1821-94).