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Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1128/AEM.70.7.4411-4414.2004 |
| Title (Primary) | Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean |
| Author | Teira, E.; Reinthaler, T.; Pernthaler, A.; Pernthaler, J.; Herndl, G.J. |
| Source Titel | Applied and Environmental Microbiology |
| Year | 2004 |
| Department | UMB |
| Volume | 70 |
| Issue | 7 |
| Page From | 4411 |
| Page To | 4414 |
| Language | englisch |
| Abstract | The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with microautoradiography, we found that Archaea are more abundant than Bacteria (42% versus 32% of 4′,6′-diamidino-2-phenylindole counts) in the deep waters of the North Atlantic and that a larger fraction of Archaea than of Bacteria takes up l-aspartic acid (19% versus 10%). |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4575 |
| Teira, E., Reinthaler, T., Pernthaler, A., Pernthaler, J., Herndl, G.J. (2004): Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by bacteria and archaea in the deep ocean Appl. Environ. Microb. 70 (7), 4411 - 4414 10.1128/AEM.70.7.4411-4414.2004 |
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