Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1016/S0014-5793(97)00480-8 |
Title (Primary) | Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP |
Author | Polte, T.; Oberle, S.; Schröder, H. |
Source Titel | FEBS Letters |
Year | 1997 |
Department | IMMU |
Volume | 409 |
Issue | 1 |
Page From | 46 |
Page To | 48 |
Language | englisch |
Keywords | Nitric oxide; Endothelial cell; Cyclic GMP; Cytoprotection; Tumor necrosis factor-a |
Abstract | In cultured endothelial cells, incubation with TNF-a (50 ng/ml) for 48 h markedly reduced viability of endothelial cells. A 6 h preincubation with Sper/NO (0.03-1 mu M) protected endothelial cells in a concentration-dependent manner and increased viability by 63% of control. The NO scavenger PTIO (30 mu M) completely abolished cytoprotection by Sper/NO. A cytoprotective effect comparable to Sper/NO was observed when preincubating the cells with 8-bromo cyclic GMP (1-10 mu M). Moreover, no protection by Sper/NO occurred in the presence of ODQ (0.1 mu M), a selective inhibitor of soluble guanylyl cyclase. Our results demonstrate that NO produces a long-term endothelial protection against cellular injury by TNF-alpha, presumably via a cyclic GMP-dependent pathway. (C) 1997 Federation of European Biochemical Societies |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=9542 |
Polte, T., Oberle, S., Schröder, H. (1997): Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP FEBS Lett. 409 (1), 46 - 48 10.1016/S0014-5793(97)00480-8 |