Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1002/(SICI)1096-9888(199711)32:11<1195::AID-JMS578>3.3.CO;2-0 |
Title (Primary) | Determination of urinary acylcarnitines by ESI-MS coupled with solid-phase microextraction (SPME) |
Author | Möder, M.; Löster, H.; Herzschuh, R.; Popp, P. |
Source Titel | Journal of Mass Spectrometry |
Year | 1997 |
Department | ANA |
Volume | 32 |
Issue | 11 |
Page From | 1195 |
Page To | 1204 |
Language | englisch |
Keywords | acylcarnitines;urine;electrospray ionization mass spectrometry;solid-phase microextraction |
Abstract | The determination of selected short-, medium- and long-chain acylcarnitines by electrospray ionization mass spectrometry (ESI-MS) is discussed. The differences in fragmentation behaviour and ionization efficiency are described in dependence on collision induced dissociation (CID) conditions and mixture composition. A new method combination, solid-phase microextraction (SPME)-ESI-MS, is introduced to characterize acylcarnitines in body fluids. This method utilizes SPME for pre-concentration of acylcarnitines from complex biological samples and ESI-MS for a selective and sensitive detection. The method is presented by standard experiments determining of acylcarnitines in aqueous solutions and urine samples from patients with secondary carnitine deficiency syndromes or related disorders. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=9497 |
Möder, M., Löster, H., Herzschuh, R., Popp, P. (1997): Determination of urinary acylcarnitines by ESI-MS coupled with solid-phase microextraction (SPME) J. Mass Spectrom. 32 (11), 1195 - 1204 10.1002/(SICI)1096-9888(199711)32:11<1195::AID-JMS578>3.3.CO;2-0 |