Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1002/cyto.a.20212 |
Title (Primary) | Analysis of living S. cerevisiae cell states - a three color approach |
Author | Achilles, J.; Harms, H.; Müller, S. |
Source Titel | Cytometry Part A |
Year | 2006 |
Department | UMB |
Volume | 69A |
Issue | 3 |
Page From | 173 |
Page To | 177 |
Language | englisch |
Keywords | multiparametric flow cytometry; Hoechst 33342; 2-NBD-glucose; viability; population dynamics; metabolic modes; Saccharomyces cerevisiae |
Abstract | Background: Biosyntheses often fluctuate with the state of the cell in the cell cycle and on the capacity of the cell to access and metabolize a carbon source, Visualization of Substrate uptake by individual cells, together with the simultaneous analysis of proliferation activity and the proportion of dead cells, facilitate reliable and quasi-online process optimization. Methods: Flow cytometry and Hoechst 33342 staining were used to follow proliferation activity of living Saccha romyces cerevisiae cells, whereas 2-NBD-glucose was employed to analyze the cells' substrate affinity. Propidium iodide was used to determine the proportion of dead cells. Calibration and verification experiments were performed with cells grown batch-wise as well as in transient state regimes. Results: A new and rapid three-color assay was developed and tested under varying microenvironmental conditions. Conclusions: Live/dead cell states and the affinity to 2-NBD-glucose vs. proliferation states were determined during respiratory and/or fermentative modes of metabolism. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2441 |
Achilles, J., Harms, H., Müller, S. (2006): Analysis of living S. cerevisiae cell states - a three color approach Cytom. Part A 69A (3), 173 - 177 10.1002/cyto.a.20212 |