Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1016/S0014-5793(99)01599-9 |
Title (Primary) | Cloning of bovine RANTES mRNA and its expression and regulation in ovaries in the periovulatory period |
Author | Aust, G.; Brylla, E.; Lehmann, I.; Kiessling, S.; Spanel-Borowski, K. |
Source Titel | FEBS Letters |
Year | 1999 |
Department | IMMU |
Volume | 463 |
Issue | 1-2 |
Page From | 160 |
Page To | 164 |
Language | englisch |
Keywords | Bovine RANTES; Ovary; Macrophage; Eosinophil |
Abstract | RANTES may be one of the chemoattractants involved in stimulating eosinophils and macrophages to migrate selectively into bovine dominant follicles and into developing corpora lutea. We sequenced a 736 bp fragment of the bovine RANTES mRNA encoding the complete protein and defined the ovarian source of RANTES mRNA. As demonstrated by competitive RT-PCR, follicle-derived macrophages showed a 100–1000 times higher RANTES mRNA level compared to unpurified granulosa cells or follicle-derived fibroblasts. By means of in situ hybridization, RANTES mRNA positive macrophages were located in the former thecal layer of the developing corpora lutea. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19201 |
Aust, G., Brylla, E., Lehmann, I., Kiessling, S., Spanel-Borowski, K. (1999): Cloning of bovine RANTES mRNA and its expression and regulation in ovaries in the periovulatory period FEBS Lett. 463 (1-2), 160 - 164 10.1016/S0014-5793(99)01599-9 |