Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/S0014-5793(99)01599-9
Title (Primary) Cloning of bovine RANTES mRNA and its expression and regulation in ovaries in the periovulatory period
Author Aust, G.; Brylla, E.; Lehmann, I.; Kiessling, S.; Spanel-Borowski, K.
Source Titel FEBS Letters
Year 1999
Department IMMU
Volume 463
Issue 1-2
Page From 160
Page To 164
Language englisch
Keywords Bovine RANTES; Ovary; Macrophage; Eosinophil
Abstract

RANTES may be one of the chemoattractants involved in stimulating eosinophils and macrophages to migrate selectively into bovine dominant follicles and into developing corpora lutea. We sequenced a 736 bp fragment of the bovine RANTES mRNA encoding the complete protein and defined the ovarian source of RANTES mRNA. As demonstrated by competitive RT-PCR, follicle-derived macrophages showed a 100–1000 times higher RANTES mRNA level compared to unpurified granulosa cells or follicle-derived fibroblasts. By means of in situ hybridization, RANTES mRNA positive macrophages were located in the former thecal layer of the developing corpora lutea.

Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19201
Aust, G., Brylla, E., Lehmann, I., Kiessling, S., Spanel-Borowski, K. (1999):
Cloning of bovine RANTES mRNA and its expression and regulation in ovaries in the periovulatory period
FEBS Lett. 463 (1-2), 160 - 164 10.1016/S0014-5793(99)01599-9