Details zur Publikation

Kategorie Textpublikation
Referenztyp Qualifizierungsarbeiten
Volltext Publikationsdokument
Titel (primär) Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures
Autor Leuthold, D.
Quelle PhD Dissertation
Erscheinungsjahr 2020
Department BIOTOX
Band/Volume 04/2020
Seite bis xxiv, 156
Sprache englisch
Keywords Zebrabärbling; Verhalten; Umweltchemikalie; Neurotoxizität; Zebrafish; Behavior; Environmental chemical; Neurotoxicity
UFZ Bestand Leipzig, Bibliothek, Reportsammlung, 00537087, 21-0157 F/E
Abstract Chemical contamination poses a risk to the aquatic environment. Chemicals can affect the biological quality of surface waters, i.e. the composition of aquatic communities. The preservation or restoration of a good ecological status of surface water bodies requires the determination of the chemical status and the assessment of its effects on biological quality. Current regulatory assessments are restricted to single priority chemicals. This approach does not account for the entirety of ecologically relevant chemicals, transformation products, or the joint effects of chemical mixtures that are ubiquitously present in water bodies.
Various toxicity tests are used to assess biological effects of environmental chemicals onaquatic organisms. However, acute toxicity tests, such as the fish embryo acute toxicity test (OECD test no. 236), lack sensitivity to detect sub-lethal chemical toxicity, particularly in the realm of chemicals that target the developing nervous system. Therefore, new approaches are required to quantify the effects of chemicals and mixtures on neurotransmission and neurodevelopment. Embryo-larval stages of zebrafish (Danio rerio) represent an excellent model system for the detection of chemical neurotoxicity, particularly during early development which represents one of the most vulnerable life stages to chemical exposure. The functionality of the zebrafish nervous system can be determined using automated behavior assays. However, key variables that influence chemical-dependent behavioral effects are not well characterized. Therefore, this thesis seeks to refine the execution, computational analyses, and applicability domain of an automated light-dark transition zebrafish behavior assay to ultimately delineate distinct behavioral responses following exposure to environmental chemicals and mixtures.
dauerhafte UFZ-Verlinkung
Leuthold, D. (2020):
Zebrafish locomotor activity as a sensitive effect-based tool for the assessment of environmental chemicals and mixtures
Dissertation, Rheinisch-Westfälische Technische Hochschule (RWTH), Fakultät für Mathematik, Informatik und Naturwissenschaften
PhD Dissertation 04/2020
Helmholtz-Zentrum für Umweltforschung - UFZ, Leipzig, xxiv, 156 pp.