P16 - Rhizosphere Microbiome
Spatiotemporal organization of the rhizosphere microbiome shaped by external drivers
The rhizosphere microbiome is of great importance to plant growth and health and provides various ecosystems services. We aim to unravel spatiotemporal organization of the rhizosphere microbiome of two corn genotypes depending on the soil (texture) and the presence of plant growth promoting bacteria (Bacillus amyloliquefaciens FZB42 gfp; Pseudomonas sp. RU47 rfp). The microbiome will be analyzed by sequencing 16S rRNA gene and ITS fragments amplified from total community DNA. Quantitative PCR and confocal laser scanning microscopy (CLSM) will be used to follow the succession of dynamic taxa (responder) and their 3D-localization on the roots. In order to gain also insights into the potential functions, bacterial isolate collections from the rhizosphere of both corn genotypes and soils will be established and their phenotypes and genotypes will be characterized. Fluorescently labeled maize plant growth promoting strains (FZB42; RU47) will be used as additional external drivers in satellite column experiments. Inoculant and responder abundance in different soil depths will be determined by qPCR and their colonization patterns along roots will be unraveled by a specific sampling technique in combination with CLSM or in situ-hybridization techniques. Applying the concept of self-organization to the rhizosphere, through the particular sampling strategies and the unique interdisciplinary approach will enable us to gain insights into the spatial spatiotemporal distribution of major microbial rhizosphere players and how their colonization patterns are shaped by external drivers.
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