Xiyu Ouyang

Current position:

PhD at Institute for environmental studies (IVM), Vrije Universiteit Amsterdam





Picture Fellow

Current research

As the number of emerging environmental pollutants is increasing dramatically nowadays, the necessity of developing a novel high resolution micro-fractionation approach to facilitate the identification as well as structure elucidation of them is urgently demonstrated.

The research within the EDA-EMERGE project is aiming to successfully develop and optimize a comprehensive two-dimensional liquid chromatography system linked to a high resolution 96-384 wells fraction collector for in vitro effect testing and mass spectrometry detection, to support the identification and monitoring of emerging toxicants in effect-directed analysis (EDA).

In practice, LCxLC-MS system was developed and methods was optimized for analyzing complex environmental samples such as effluent and sediment sample. Straight forward fractionation was established after the LCxLC separation. For toxicity testing, Zebrafish (Danio rerio) embryo toxicity test was performed to screen for the active fractions.

Besides, a high throughput site-specific fluorescence assay using a fluorescein-thyroxin conjugate was developed to study thyroid hormone disruption in the environment.


  • HPLC
  • LCxLC
  • LC-MS
  • Toxicology

  • Environmental chemistry
  • Spectroscopy (NMR, UV, FTIR, etc)
  • Organic chemistry 

Research interests

  • Hyphenated technique in analytical chemistry
  • Two dimensional chromatography
  • EDA study of complex environmental samples
  • Development of High throughput bioassay

Curriculum vitae

Since 2012

PhD at Institute for environmental studies (IVM), Vrije Universiteit Amsterdam
Topic of PhD: “Development of a novel high resolution micro-fractionation approach based on comprehensive LCxLC linked to accurate mass spectrometry".


Master of Science in Advanced Spectroscopy in Chemistry
University of Leipzig, Germany/University of Helsinki, Finland with Erasmus Mundus fellowship (2 year full scholarship provided by European Commission)
Title of thesis “Study the oxidation process of phospholipids with different head groups and corresponding lysophospholipids generation as disease markers induced by an excess of HOCl based on qualitative and quantitative analysis of MALDI-TOF MS”.


Bachelor of Science in Chemistry, Wuhan University, China
Title of thesis: “Characterization of NOx removal in industrial gaseous waste with diethylenetriamine cobalt solution by spectroscopic methods”.


Ouyang, X., et al., 2014. Online LCxLC-ToF MS for Effect-Directed Analysis in Effluent and Surface Water. Oral presentation, International Mass Spectrometry Conference (IMSC 2014), Geneva Switzerland.

Ouyang, X., et al., 2014. Comprehensive Multi-dimensional Liquid Chromatography for Effect-directed Analysis in Effluents and Surface Water. Oral presentation, HPLC 2014, New Orleans, USA.

Ouyang, X., et al., 2013. Comprehensive multi-dimensional liquid chromatography (LCxLC) approaches for application in effect-directed analysis. Poster presentation, HPLC 2013, Amsterdam, The Netherlands.

Ouyang, X., et al., 2012. Comprehensive two dimensional liquid chromatography approaches for separation of complex contaminants with bio-testing and mass spectrometry. Poster presentation, NORMAN workshop, Amsterdam, The Netherlands.


Ouyang, X., Leonards, P., Legler, J., van der Oost, R., De Boer, J., Lamoree, M., 2015. Comprehensive two-dimensional liquid chromatography coupled to high resolution time of flight mass spectrometry for chemical characterization of sewage treatment plant effluents. Journal of Chromatography A, 1380, 139-145.

Werner Brack, Selvan Govender, Tobias Schulze, Martin Krauss, Meng Hu, Melis Muz, Juliane Hollender, Kristin Schirmer, Jennifer Schollee, Anita Hidasi, Jaroslav Slobodnik, Zuzana Rabova, Selim Ait-Aissa, Manoj Sonavane, Mario Carere, Marja Lamoree, Pim Leonards, Sara Tufi, Xiyu Ouyang, Merijn Schriks, Kevin Thomas, Ana Catarina de Almeida, Jean Froment, Monika Hammers-Wirtz, Marijan Ahel, Sanja Koprivica, Henner Hollert, Thomas-Benjamin Seiler, Carolina Di Paolo, Andrew Tindall and Petra Spirhanzlova, 2013. EDA-EMERGE: an FP7 initial training network to equip the next generation of young scientists with the skills to address the complexity of environmental contamination with emerging pollutants, Environmental Sciences Europe, 25-18.


EDA-EMERGE Newsletter



Marie Curie ITN EU-FP7 project