Chapter 13
Design of test batteries and interpretation of bioassay results
You and your work group have been selected to lead a project that will study the quality of tap water in your city over a year using in vitro bioassays. You are first asked to create a test battery of bioassays that you can use to efficiently carry out this surveillance. Which assays do you choose? Explain your decision.
Explain the principle of “Effect-directed Analysis” (EDA). For which category of bioassays and for which samples should EDA be used?
In the Excel sheet “Exercise SRbaseline” you find underlying data from Escher and Neale (2021), DOI: 10.1002/etc.4944 from the PPARγ assay.
The table shows effect concentrations and IC10baseline concentrations from the PPARγ assay. Calculate the SRbaseline of all chemicals and decide if the PPARγ assay is a category 1 or 2 assay. Please explain your decision.
Explain how effect-based trigger values (EBTs) can be derived for drinking water and surface water. What are the input parameters and how are they processed? What is the difference in the derivation of EBTs for Category 1 and Category 2 bioassays?