"Making sense out of (bio)analytical data in chemical mixture assessment"

Registration is now open for the next course that will take place in February 2025.

This course is designed to introduce the fundamentals of in vitro assays.

It is a self-paced online course consisting of 4 modules and one introduction meeting on 27 January 2025. Each module focuses on a different aspect of in vitro assays.

Module 1 provides basic knowledge for cell-based bioassays.
Module 2 deals with mixtures and environmental applications of in vitro bioassays.
Module 3 high-lights the importance of quality control and quality assurance of bioassays – after all: Your results are only as good as your methods.
Module 4 covers applications of in vitro assays for the risk assessment of chemicals.

The course can be taken as a 1 to 4 day course – if you do not wish to take the entire course, you can specify this in your registration.

1 day: module 1
2 days: modules 1 & 2
3 days: modules 1 & 2 & 3 or 1 & 2 & 4
4 days: modules 1 & 2 & 3 & 4

The course is based on the book "Bioanalytical Tools in Water Quality Assessment" which you can access by clicking on the link.

Each of the modules consists of some required reading, some recommended reading, exercises and an online session for discussions. In the table below you find these details for each module.

Module Book
(not required)
available at
Exercises Discussion

on Zoom

Introduction to cell-based bioassays 1   Unit 1.mp4 Q&a chap. 1 Monday 27/01/2025,
9-10 am CET
1. Basic knowledge for cell-based bioassays          
I) Principles of cell based bioassays
II) Concentration-response modelling

4, 5, 6 Unit 10.mp4 
Unit 7.mp4
Q&A chap. 10

Q&A chap. 7
Thursday 06/02/2025,
9-11 am CET
2. Mixtures and environmental applications of in vitro bioassays          
I) Mixtures

II) Design of test batteries and interpretation of bioassay results


Unit 8.mp4

Unit 13.mp4
Q&A chap. 8

Q&A chap. 13
Thursday 13/02/2025,
9-11 am CET
3. Your results are only as good as your methods          
I) Quality control/quality assurance of bioassays 

II) Sampling and dosing



Q&A chap. 11

Q&A chap. 12
Thursday 20/02/2025,
9-11 am CET
4. Application of in vitro assays for the risk assessment of chemicals          
I) Risk assessment of chemicals
II) Water Quality Assessment and whole effluent toxicity testing
III) In vitro assays in risk assessment





Q&A chap. 2

Q&A chap. 3 


Q&A chap. 9
Thursday 27/02/2025,
9-11 am CET

The exercises for each chapter can be found on this website on the corresponding chapter pages. For some chapters there is also a presentations and/or a video available which can also be found on those pages.

The course is held by Prof. Beate Escher, the first author of the book "Bioanalytical Tools in Water Quality Assessment". Prof. Escher presently teaches at the University of Tübingen and at the Swiss Federal Institute of Technology in the areas of environmental chemistry, toxicology and risk assessment, which are also her research area.

To register for the course, please send an email to . You will then be given the access code for the discussion meetings on zoom.

After this course, participants have a basic understanding of the fundamentals and the underlying principles of in vitro assays and their application in environmental monitoring.