Hyphenation of cell‐based assays with microfractionation procedures (SC9)
Course description:
The development of microfractionation procedures to obtain fractions containing relatively simple mixtures of compounds for in vitro testing and high resolution mass spectrometry is one of the major challenges of high throughput Effect-Directed Analysis. Microfractionation is expected to lead to an improved bioactivity-to-identity correlation of known and unknown compounds in environmental matrices, resulting in a wider acceptance of EDA as a tool for e.g. the investigative monitoring of water quality.
The SC9 was a 2 day EDA-EMERGE training course on hyphenation of cell‐based assays with microfractionation procedures. Said training course focused on teaching the fundamentals of developing microfractionation procedures to obtain fractions containing relatively simple mixtures of compounds for in vitro testing and high resolution mass spectrometry.
The course included lectures on topics such as in vitro assays currently used in EDA, toxicity profiling, ‐omics approaches, DNA array fingerprinting as well as lectures on the use of LCxLC, combining fractionation with bioassays and chemical identification and on automatisation of the fractionation and bioassay axis. In an additional practical part of the course, the participants were introduced in LCxLC applications in the laboratory.
Date:
21-22 January 2014
Venue:
The course took place at VU-IVM in Amsterdam, Netherlands
