Microarray Data - Transcriptional expression - Livertissue - 48h Exposure of adult fathead minnows Small Streams US 0 8332

Project
POF3 - T42 - Exposome - ProxiesOfTheEcoexposome - 10STREAMS
Description
The zip file contains 71 txt-files with output of Agilent Microarray gene expression analysis
Platform: GPL9248

File names encode site and running number:
[Site-ID]-[RunNr].txt, for example 15EM014-36 presents site identified with 15EM14 and was measured as 36th microarray in the laboratory.

Streamsites:
Samples of streamwater were collected in September and October 2014.In total 146 chemicals were measured (with duplicated measurements, 142 unique compounds)

Exposure experiments:
7-8 Adult and sexually mature fish (Fathead minnow - Pimephales promelas) were exposed for 48h to either streamwaters of a specific site or clean control water (filtered, ultraviolet lighttreated Lake Superior water). Flow rates, photoperiod, feeding, water temperature and dissolved oxygen levels were kept the same for all fish exposures. Microarray-based analysis of liver gene expression was conducted using a custom 60K feature fathead minnow DNA microarray following previously published protocols (Martinović-Weigelt, D., et al., Transcriptomic Effects-Based Monitoring for Endocrine Active Chemicals: Assessing Relative Contribution of Treated Wastewater to Downstream Pollution. Environ Sci Technol, 2014. 48(4): p. 2385-2394.)
Size
593,0 MB
License and Terms of Use
LICENSE and TERMS OF USE
Data quality
quality assured data
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Metadata Catalogues
Dublin Core
URI
http://www.ufz.de/record/dmp/archive/8332
Subject
Raw Microarray transcriptional gene expression data
Coverage
Small Streams Minnesota, US
Type of data
dataset (structured data in a defined format)
Data quality
quality assured data
Status of data
raw data
Data format
zip
txt
Creators
Krämer, Stefan
Contributors
Krämer, Stefan
Dalma Martinovic-Weigelt
Publisher
Helmholtz-Zentrum für Umweltforschung
Rights holders
Helmholtz-Zentrum für Umweltforschung
Source/Origin
Dalma Martinovic-Weigelt, University of St.Thomas (St.Paul, MN, US)
Contact
Contact content: Stefan Kraemer (stefan.kraemer@ufz.de)
Contact data generation: Dalma Martinovic-Weigelt (mart6831@stthomas.edu)
Time or Period of creation
28.03.2018
Language of the content
English
Update policy
stored data will not be extended
Access permissions
limited access