2023 ProVis Y. Davoudpour et al. - Tracking deuterium uptake in hydroponically grown Zea mays roots using correlative helium ion microscopy and confocal Raman micro-spectroscopy 0 13952

Projekt
POF3 - T15 - Sustainable Biotechnology and Bioeconomy - ProVIS - SEM/HIM Data
Beschreibung
Description is the abstract of publication in Plant Methods
Y. Davoudpour et al. 2023, https://doi.org/10.1186/s13007-023-01040-y

Background:
Investigations into the growth and self-organization of plant roots is subject to fundamental and
applied research in various areas such as botany, agriculture, and soil science. The growth activity of the plant
tissue can be investigated by isotope labeling experiments with heavy water and subsequent detection of the
deuterium in non-exchangeable positions incorporated into the plant biomass. Commonly used analytical methods
to detect deuterium in plants are based on mass-spectrometry or neutron-scattering and they either suffer from
elaborated sample preparation, destruction of the sample during analysis, or low spatial resolution. Confocal Raman
micro-spectroscopy (CRM) can be considered a promising method to overcome the aforementioned challenges.
The substitution of hydrogen with deuterium results in the measurable shift of the CH-related Raman bands. By
employing correlative approaches with a high-resolution technique, such as helium ion microscopy (HIM), additional
structural information can be added to CRM isotope maps and spatial resolution can be further increased. For that, it
is necessary to develop a comprehensive workflow from sample preparation to data processing.

Results:
A workflow to prepare and analyze roots of hydroponically grown and deuterium labeled Zea mays by
correlative HIM-CRM micro-analysis was developed. The accuracy and linearity of deuterium detection by CRM were
tested and confirmed with samples of deuterated glucose. A set of root samples taken from deuterated Zea mays
in a time-series experiment was used to test the entire workflow. The deuterium content in the roots measured by
CRM was close to the values obtained by isotope-ratio mass spectrometry. As expected, root tips being the most
actively growing root zone had incorporated the highest amount of deuterium which increased with increasing
time of labeling. Furthermore, correlative HIM-CRM analysis allowed for obtaining the spatial distribution pattern
of deuterium and lignin in root cross-sections. Here, more active root zones with higher deuterium incorporation
showed less lignification.

Conclusions
We demonstrated that CRM in combination with deuterium labeling can be an alternative and reliable
tool for the analysis of plant growth. This approach together with the developed workflow has the potential to be
extended to complex systems such as plant roots grown in soil.
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Metadaten Kataloge
Dublin Core
URI
https://www.ufz.de/record/dmp/archive/13952
Thema
Zea mays roots
deuterium labelling as growth marker
helium ion microscopy
confocal Raman micro-spectroscopy
EA-Cr-HTC-IRMS
Correlative Microscopy
Correlia
Veröffenlichungsdatum
03.07.2023
Geltungsbereich
Helmholtz Centre for Environmental Research (UFZ) GmbH
ProVIS - Centre for Chemical Microscopy
Permoserstraße 15
04318 Leipzig
Germany
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Urheber
Schmidt, Matthias
Davoudpour, Yalda
Beitragende
Davoudpour, Yalda
Schmidt, Matthias
Kümmel, Steffen
Musat, Niculina
Richnow, Hans Hermann
Herausgeber
Helmholtz-Centre for Environmental Research
Rechteinhaber
Helmholtz-Centre for Environmental Research
Quelle/Ursprung
DOI: 10.1186/s13007-023-01040-y
Hinweis zur Zitation
please cite the DOI of this data as well as the publication in Plant Methods, DOI: 10.1186/s13007-023-01040-y
Kontakt
matthias.schmidt@ufz.de
yalda.davoudpour@ufz.de
steffen.kuemmel@ufz.de
Zeitpunkt oder -bereich der Erzeugung der Daten
01.11.2019 - 31.10.2022
Sprache des Inhalts
Englisch
Version
1.0
Zielgruppe
plant pysiologists
microscopists
soil scientists
scientists with background in isotope labelling
scientists with background in correlative microscopy
Besitzänderung
none
Lehrmethode
publication in Plant Methods (Davoudpour et al. 2023), DOI: 10.1186/s13007-023-01040-y
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none
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