Data for "The potential of multispectral imaging flow cytometry for environmental monitoring" 0 12577

Project
POF3 - T11 - Emerging ecosystems - PolDiv/iCyt
Description
Raw data and images related to the publication: "The potential of multispectral imaging flow cytometry for environmental monitoring" https://doi.org/10.1002/cyto.a.24658
Figure 2: Allergen staining of birch (Betula sp.) pollen. Betula sp. pollen (40x magnification) collected in Leipzig, Germany with their autofluorescence (A) and labelled with a polyclonal BETV1A antibody (FITC), IgG, Rabbit (Biozol, Eching, Germany) (B). Fluorescence was measured with Ex. 488 nm/Em. 528/65 nm.
Figure 3: Pollen from honey samples collected at Fürstenrieder Schloss (Munich, Germany). Multispectral microscopic images (40x magnification) of one particle in each row for different channels. Brightfield channels (Ch01/ Ch09), fluorescence image channels of different spectral ranges: Ch02 - Ex. 488 nm/Em. 528/65 nm, Ch03 - Ex. 488 nm/Em. 577/35 nm, Ch04 - Ex. 488 nm/Em. 610/30 nm, Ch05 - Ex. 488 nm/Em. 702/85 nm, Ch07 – Ex. 561 nm/Em. 457/45 nm, Ch08 – Ex. 561 nm/Em. 537/65 nm, Ch10 – Ex. 561 nm/Em. 610/30 nm, Ch11 – Ex. 561 nm/Em. 702/85 nm, Ch12 – Ex. 561 nm/Em. 762/35 nm and scatter channel (Ch06 Ex. 785 nm). Sample preparation was according to DIN 10760.
Figure 4: ~ 6000-year-old pollen from a sediment core of Lake Rauchuagytgyn, North-East Siberia. Multispectral microscopic images (40x magnification) of one particle in each row for different channels. Brightfield channels (Ch01/ Ch09), fluorescence image channels of different spectral ranges: Ch02 - Ex. 488 nm/Em. 528/65 nm, Ch03 - Ex. 488 nm/Em. 577/35 nm, Ch04 - Ex. 488 nm/Em. 610/30 nm, Ch05 - Ex. 488 nm/Em. 702/85 nm, Ch07 – Ex. 561 nm/Em. 457/45 nm, Ch08 – Ex. 561 nm/Em. 537/65 nm, Ch10 – Ex. 561 nm/Em. 610/30 nm, Ch11 – Ex. 561 nm/Em. 702/85 nm, Ch12 – Ex. 561 nm/Em. 762/35 nm and scatter channel (Ch06 Ex. 785 nm).
Figure 5: Microplastic particles. Multispectral microscopic images (40x magnification) of one particle in each row for different channels. Brightfield channels (Ch01/ Ch09), fluorescence image channels of different spectral ranges: Ch02 - Ex. 488 nm/Em. 528/65 nm, Ch03 - Ex. 488 nm/Em. 577/35 nm, Ch04 - Ex. 488 nm/Em. 610/30 nm, Ch05 - Ex. 488 nm/Em. 702/85 nm, Ch07 – Ex. 561 nm/Em. 457/45 nm, Ch08 – Ex. 561 nm/Em. 537/65 nm, Ch10 – Ex. 561 nm/Em. 610/30 nm, Ch11 – Ex. 561 nm/Em. 702/85 nm, Ch12 – Ex. 561 nm/Em. 762/35 nm and scatter images (Ch06 Ex. 785 nm) of polyamide particles used in a microplastic research project (MikroPlaTaS https://bmbf-plastik.de/de/verbundprojekt/mikroplatas).
Figure 6: Algae and cyanobacteria. Some exemplary algae as (A) Pediastrum biradiatum, B) Cryptomonas ovata and a cyanobacterium C) Anabaena flos-aquae being relevant for the monitoring of the EU Water Framework Directive. Multispectral microscopic images (40x magnification) of one particle in each row for different channels. Brightfield channels (Ch02/ Ch08), fluorescence image channels of different spectral ranges: Ch03 - Ex. 488 nm/Em. 577/35 nm, Ch04 - Ex. 488 nm/Em. 610/30 nm, Ch05 - Ex. 488 nm/Em. 702/85 nm, Ch09 – Ex. 561 nm/Em. 582/35 nm, Ch10 – Ex. 561 nm/Em. 610/30 nm, Ch11 – Ex. 561 nm/Em. 702/85 nm and scatter channel (Ch12 Ex. 785 nm).
Figure 7: Bryophyte spores. Multispectral microscopic images (40x magnification) of one particle in each row for different channels. Brightfield channels (Ch01/ Ch09), fluorescence image channels of different spectral ranges: Ch02 - Ex. 488 nm/Em. 528/65 nm, Ch03 - Ex. 488 nm/Em. 577/35 nm, Ch04 - Ex. 488 nm/Em. 610/30 nm, Ch05 - Ex. 488 nm/Em. 702/85 nm and scatter channel (Ch6 Ex. 785 nm) of the species A) Dicranum sp. and B) Rhytidiadelphus sp. collected at Lotharpfad, Black Forest National Park, Germany.
Figure 8: Plant cells. Multispectral microscopic images (40x magnification) of one particle in each row for different channels. Brightfield channels (Ch01/ Ch09), fluorescence image channels of different spectral ranges: Ch02 - Ex. 488 nm/Em. 528/65 nm, Ch03 - Ex. 488 nm/Em. 577/35 nm, Ch04 - Ex. 488 nm/Em. 610/30 nm, Ch05 - Ex. 488 nm/Em. 702/85 nm, Ch07 – Ex. 561 nm/Em. 457/45 nm, Ch08 – Ex. 561 nm/Em. 537/65 nm, Ch10 – Ex. 561 nm/Em. 610/30 nm, Ch11 – Ex. 561 nm/Em. 702/85 nm, Ch12 – Ex. 561 nm/Em. 762/35 nm and scatter channel (Ch06 Ex. 785 nm) of Centaurea sp. cells, treated with RNAse and stained with Sytox (Ch02).
Figure 9: Plant starch granules. Multispectral microscopic images (40x magnification) of one particle in each row for different channels. Brightfield channels (Ch01/ Ch09), fluorescence image channels of different spectral ranges: Ch02 - Ex. 488 nm/Em. 528/65 nm, Ch03 - Ex. 488 nm/Em. 577/35 nm, Ch04 - Ex. 488 nm/Em. 610/30 nm, Ch05 - Ex. 488 nm/Em. 702/85 nm, Ch07 – Ex. 561 nm/Em. 457/45 nm, Ch08 – Ex. 561 nm/Em. 537/65 nm, Ch10 – Ex. 561 nm/Em. 610/30 nm, Ch11 – Ex. 561 nm/Em. 702/85 nm, Ch12 – Ex. 561 nm/Em. 762/35 nm and scatter images (Ch06 Ex. 785 nm) of starch granules from Musa sp. (A) and Zea mays (B).
DOI
https://doi.org/10.48758/ufz.12577
Cite as (APA)
Dunker, S. (2022). Data for "The potential of multispectral imaging flow cytometry for environmental monitoring". Helmholtz-Zentrum für Umweltforschung. https://doi.org/10.48758/UFZ.12577
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2,6 GB
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Metadata Catalogues
Dublin Core
URI
https://www.ufz.de/record/dmp/archive/12577
DOI
10.48758/ufz.12577
Subject
Data multispectral imaging flow cytometry
Publication Date
14.04.2022
Type of data
collection of various data
Data quality
quality assured data
Creators
Dunker, Susanne
Publisher
Helmholtz-Zentrum für Umweltforschung
Rights holders
Helmholtz-Zentrum für Umweltforschung
Contact
Susanne Dunker
Time or Period of creation
01.12.2016 - 30.03.2022
Language of the content
German
Update policy
stored data will not be extended
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limited access