Doctoral researcher skilled in: Ratiometric optical sensing; Single-cell analysis; Advanced microscopic imaging and image analysis.
My current research interest includes:
Optical oxygen sensing
Here, I develop platforms to optically follow the spatiotemporal oxygen profiles in micro-environments such as mycosphere. Combining advanced time-lapse microscopy and molecular approaches, I also resolve in vivo effects of microscale oxygen on the bacterial life, e.g. bacterial germination, growth, metabolic activity and spatial dispersal.
Optical pH sensing using a novel whole-cell pH bioreporter
Here, I mainly focus on developing and application of a whole-cell pH bioreporter to report environmental pH at single-cell level. A ratiometric, florescence-based bioreporter has been developed and applied to follow spatiotemporal pH dynamics in the micro-environments such as mycosphere.
pH & oxygen sensing at single-cell level
Here, I develop micro-platforms (i.e. microfluidic devices) to resolve pH/oxygen and their effects on bacterial activity at single-cell level. Time-lapse microscopy, micrometre-sized oxygen-sensitive beads and single-cell pH bioreporters are used to measure the environmental pH/oxygen in the hyphal liquid water (‘fungal highways’) and their effects on bacterial motility.
Bi-Jing Xiong, Christian Dusny, Lin Wang, Jens Appel, Kristin Lindstaedt, Dietmar Schlosser, Hauke Harms, and Lukas Y. Wick, 2021, Illuminate the hidden: in vivo mapping of microscale pH in the mycosphere using a novel whole-cell biosensor, ISME commons.
Bi-Jing Xiong, Youchi Zhang, Yanwei Hou, Hans Peter H. Arp, Brian J. Reid, Chao Cai, Enhanced biodegradation of PAHs in historically contaminated soil by M. gilvum inoculated biochar, 2017, Chemosphere.
Chao Cai, Bi-Jing Xiong, Youchi Zhang, Xinhu Li, Luis M. Nunes, Critical comparison of soil pollution indices for assessing contamination with toxic metals, 2015, Water, air & soil pollution.
Patent: Chao Cai, Bi-Jing Xiong, Youchi Zhang, Yongguan zhu, 2017, Remediation of PAHs contaminated soil using biochar-microbe composite, patent No. Zl201510390223.8