Dr. Luis Filipe Morgado Rosa
CV / Scientific Career
During my PhD I have worked in production and purification of a human recombinant membrane haemic enzyme (CYP3A4) in E.coli using bio-reactors, optimizing the productivity of P450 protein, and developed a simplified purification process from membrane fractions using FPLC. I have also acquired proficiency on several techniques beyond those that are most common in an enzyme production and purification laboratory: Electrochemistry techniques, (CV, chronoamperometry), Circular dichroism, HPLC-ESI-MS, Fluorescence emission, Light Scattering, Zeta-sizing, amongst others. Plasmid DNA cloning and manipulation, and RP-HPLC are also techniques I have come to master during this time.
I am also very proficient with sensors and electronics. I have used CYP3A4 to create a biosensor using QCM and electrochemical transduction, and have developed during the PhD, innovative signal acquisition techniques for QCM using real time impedance analysis using complex Impedance analysis.
The objective of my first Post-Doc fellow was to set up and perform diverse electrochemical analytic techniques to the multi heme cytochromes the group had previously produced and purified, and also to start up and maintain investigation on Microbial Fuell Cells using pure cultures of dissimilatory respiring organisms from Shewanwlla, Geobacter, Dessulfuromonas and other microbial families using minimal media. In the meantime I have also learned how to produce and purify one of the several multi heme cytochromes mastered in the lab (mtra).
One of my latest interests is also numerical modeling of mass transport, diffusion, convection and reaction in micro and macro-fluidic settings using finite element modeling with Comsol Multiphysics (previously known as FEMLAB) software.
Co-operations / Projects
Are detailed at the website of the working group.