Research Focus:
The overall productivity of any bioprocess is determined by the stability and the efficiency of the biocatalyst, the amount of product produced in a given reaction volume and the yield of product recovery. We use cyanobacteria for splitting water into hydrogen and oxygen driven by energy of the light reaction of photosynthesis. The turnover number of the biocatalyst (kcat in s-1) and its stability over time are determining the amount of hydrogen produced in a given reaction volume (mass of H2 formed per mass of biocatalyst, the total turnover number). This again is limited by the quantum efficiency of the reaction network and thermodynamic and metabolic constraints. Ideally, product recovery is integrated in a continuous production process. We integrate metabolic engineering of natural photosynthesis with continuously operated microreactors based on catalytic biofilms as biocatalysts with membrane technology for maximizing surface to volume ratios and mass transfer rates.
Head:
PhD-Student:
Technicians:
Index:
2023 (1)2022 (6)
2021 (9)
2020 (3)
2019 (12)
2018 (5)
2017 (11)
2016 (10)
2015 (17)
You could use our publication index for further requests.
2023 (1)
- Garmasukis, R., Hackl, C., Dusny, C., Elsner, C., Charvat, A., Schmid, A., Abel, B. (2023):
Cryo-printed microfluidics enable rapid prototyping for optical-cell analysis
Microfluid. Nanofluid. 27 , art. 5
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2022 (6)
- Bertelmann, C., Mock, M., Koch, R., Schmid, A., Bühler, B. (2022):
Hydrophobic outer membrane pores boost testosterone hydroxylation by cytochrome P450 BM3 containing cells
Front. Catal. 2 , art. 887458
full text (doi) - Bozan, M., Schmid, A., Bühler, K. (2022):
Evaluation of self-sustaining cyanobacterial biofilms for technical applications
Biofilm 4 , art. 100073
full text (doi) - Brandenburg, F., Klähn, S., Schmid, A., Krömer, J.O. (2022):
Produktion von Aminosäurederivaten in Cyanobakterien
Biospektrum 28 (3), 341 - 343
full text (doi) - Grund, M., Jakob, T., Toepel, J., Schmid, A., Wilhelm, C., Bühler, B. (2022):
Heterologous lactate synthesis in Synechocystis sp. strain PCC 6803 causes a growth condition-dependent carbon sink effect
Appl. Environ. Microb. 88 (8), e00063-22
full text (doi) - Tüllinghoff, A., Uhl, M.B., Nintzel, F.E.H., Schmid, A., Bühler, B., Toepel, J. (2022):
Maximizing photosynthesis-driven Baeyer-Villiger oxidation efficiency in recombinant Synechocystis sp. PCC6803
Front. Catal. 1 , art. 780474
full text (doi) - Wink, K., van der Loh, M., Hartner, N., Polack, M., Dusny, C., Schmid, A., Belder, D. (2022):
Quantification of biocatalytic transformations by single microbial cells enabled by tailored integration of droplet microfluidics and mass spectrometry
Angew. Chem.-Int. Edit. 61 (29), e202204098
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2021 (9)
- Brandenburg, F., Theodosiou, E., Bertelmann, C., Grund, M., Klähn, S., Schmid, A., Krömer, J.O. (2021):
Trans-4-hydroxy-L-proline production by the cyanobacterium Synechocystis sp. PCC 6803
Metab. Eng. Commun. 12 , e00155
full text (doi) - Bühler, K., Bühler, B., Klähn, S., Krömer, J.O., Dusny, C., Schmid, A. (2021):
Biocatalytic production of white hydrogen from water using cyanobacteria
In: Rögner, M. (ed.)
Photosynthesis: Biotechnological applications with microalgae
De Gruyter, Berlin ; Boston, p. 279 - 306
full text (doi) - Bühler, K., Krömer, J.O., Klähn, S., Bühler, B., Dusny, C., Schmid, A. (2021):
Weißer Wasserstoff made in Leipzig
Biospektrum 27 (3), 335
full text (doi) - Dusny, C., Schmid, A. (2021):
The metabolic flux probe (MFP)—Secreted protein as a non-disruptive information carrier for 13C-based metabolic flux analysis
Int. J. Mol. Sci. 22 (17), art. 9438
full text (doi) - Lai, B., Schneider, H., Tschörtner, J., Schmid, A., Krömer, J.O. (2021):
Inside front cover image, Volume 118, Number 7, July 2021
Biotechnol. Bioeng. 118 (7), ii - ii
full text (doi) - Lai, B., Schneider, H., Tschörtner, J., Schmid, A., Krömer, J.O. (2021):
Technical-scale biophotovoltaics for long-term photo-current generation from Synechocystis sp. PCC6803
Biotechnol. Bioeng. 118 (7), 2637 - 2648
full text (doi) - Lupacchini, S., Appel, J., Stauder, R., Bolay, P., Klähn, S., Lettau, E., Adrian, L., Lauterbach, L., Bühler, B., Schmid, A., Toepel, J. (2021):
Rewiring cyanobacterial photosynthesis by the implementation of an oxygen-tolerant hydrogenase
Metab. Eng. 68 , 199 - 209
full text (doi) - Salamanca, D., Bühler, K., Engesser, K.-H., Schmid, A., Karande, R. (2021):
Whole-cell biocatalysis using the Acidovorax sp. CHX100 Δ6HX for the production of ω-hydroxycarboxylic acids from cycloalkanes
New Biotech. 60 , 200 - 206
full text (doi) - Welter, E.S., Kött, S., Brandenburg, F., Krömer, J.O., Goepel, M., Schmid, A., Gläser, R. (2021):
Figures of merit for photocatalysis: Comparison of NiO/La-NaTaO3 and Synechocystis sp. PCC 6803 as a semiconductor and a bio-photocatalyst for water splitting
Catalysts 11 (11), art. 1415
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2020 (3)
- Mock, M., Schmid, A., Bühler, K. (2020):
Directed reaction engineering boosts succinate formation of Synechocystis sp. PCC 6803_Δsll1625
Biotechnol. J. 15 (11), art. 2000127
full text (doi) - Schirmer, M., Wink, K., Ohla, S., Belder, D., Schmid, A., Dusny, C. (2020):
Conversion efficiencies of a few living microbial cells detected at a high throughput by droplet-based ESI-MS
Anal. Chem. 92 (15), 10700 - 10708
full text (doi) - Willrodt, C., Gröning, J.A.D., Nerke, P., Koch, R., Scholtissek, A., Heine, T., Schmid, A., Bühler, B., Tischler, D. (2020):
Highly efficient access to (S)-sulfoxides utilizing a promiscuous flavoprotein monooxygenase in a whole-cell biocatalyst format
ChemCatChem 12 (17), 4664 - 4671
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2019 (12)
- David, C., Schmid, A., Bühler, K. (2019):
Cellular physiology controls photoautotrophic production of 1,2‐propanediol from pools of CO2 and glycogen
Biotechnol. Bioeng. 116 (4), 882 - 892
full text (doi) - Dusny, C., Lohse, M., Reemtsma, T., Schmid, A., Lechtenfeld, O.J. (2019):
Quantifying a biocatalytic product from a few living microbial cells using microfluidic cultivation coupled to FT-ICR-MS
Anal. Chem. 91 (11), 7012 - 7018
full text (doi) - Grund, M., Jakob, T., Wilhelm, C., Bühler, B., Schmid, A. (2019):
Electron balancing under different sink conditions reveals positive effects on photon efficiency and metabolic activity of Synechocystis sp. PCC 6803
Biotechnol. Biofuels 12 , art. 43
full text (doi) - Halan, B., Tschörtner, J., Schmid, A. (2019):
Generating electric current by bioartificial photosynthesis
In: Harnisch, F., Holtmann, D. (eds.)
Bioelectrosynthesis
Adv. Biochem. Eng. Biotechnol. 167
Springer Nature, p. 361 - 393
full text (doi) - Heuschkel, I., Hoschek, A., Schmid, A., Bühler, B., Karande, R., Bühler, K. (2019):
Mixed-trophies biofilm cultivation in capillary reactors
MethodsX 6 , 1822 - 1831
full text (doi) - Heuschkel, I., Hoschek, A., Schmid, A., Bühler, B., Karande, R., Bühler, K. (2019):
Data on mixed trophies biofilm for continuous cyclohexane oxidation to cyclohexanol using Synechocystis sp. PCC 6803
Data Brief 25 , art. 104059
full text (doi) - Hoschek, A., Bühler, B., Schmid, A. (2019):
Stabilization and scale‐up of photosynthesis‐driven ω‐hydroxylation of nonanoic acid methyl ester by two‐liquid phase whole‐cell biocatalysis
Biotechnol. Bioeng. 116 (8), 1887 - 1900
full text (doi) - Hoschek, A., Heuschkel, I., Schmid, A., Bühler, B., Karande, R., Bühler, K. (2019):
Mixed-species biofilms for high-cell-density application of Synechocystis sp. PCC 6803 in capillary reactors for continuous cyclohexane oxidation to cyclohexanol
Bioresour. Technol. 282 , 171 - 178
full text (doi) - Hoschek, A., Toepel, J., Hochkeppel, A., Karande, R., Bühler, B., Schmid, A. (2019):
Light‐dependent and aeration‐independent gram‐scale hydroxylation of cyclohexane to cyclohexanol by CYP450 harboring Synechocystis sp. PCC 6803
Biotechnol. J. 14 (8), art. 1800724
full text (doi) - Mock, M., Schmid, A., Bühler, K. (2019):
Photoautotrophic production of succinate via the oxidative branch of the tricarboxylic acid cycle influences glycogen accumulation in Synechocystis sp. PCC 6803
Algal Res. 43 , art. 101645
full text (doi) - Ütkür, F.Ö., Schmid, A., Bühler, B. (2019):
Anaerobic C-H oxyfunctionalization: Coupling of nitrate reduction and quinoline hydroxylation in recombinant Pseudomonas putida
Biotechnol. J. 14 (8), art. 1800615
full text (doi) - Volmer, J., Lindmeyer, M., Seipp, J., Schmid, A., Bühler, B. (2019):
Constitutively solvent-tolerant Pseudomonas taiwanensis VLB120ΔCΔttgV supports particularly high styrene epoxidation activities when grown under glucose excess conditions
Biotechnol. Bioeng. 116 (5), 1089 - 1101
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2018 (5)
- David, C., Schmid, A., Adrian, L., Wilde, A., Bühler, K. (2018):
Production of 1,2-propanediol in photoautotrophic Synechocystis is linked to glycogen turn-over
Biotechnol. Bioeng. 115 (2), 300 - 311
full text (doi) - Hoschek, A., Schmid, A., Bühler, B. (2018):
In situ O2 generation for biocatalytic oxyfunctionalization reactions
ChemCatChem 10 (23), 5366 - 5371
full text (doi) - Karande, R., Salamanca, D., Schmid, A., Buehler, K. (2018):
Biocatalytic conversion of cycloalkanes to lactones using an in-vivo cascade in Pseudomonas taiwanensis VLB120
Biotechnol. Bioeng. 115 (2), 312 - 320
full text (doi) - Oehling, V., Klaassen, P., Frick, O., Dusny, C., Schmid, A. (2018):
L-Arabinose triggers its own uptake via induction of the arabinose-specific Gal2p transporter in an industrial Saccharomyces cerevisiae strain
Biotechnol. Biofuels 11 , art. 231
full text (doi) - Schaffer, S., Schmid, A. (2018):
Aminolauric acid: From design of a multistep synthesis route to large‐scale process development
Chem. Ing. Tech. 90 (9), 1334 - 1334
full text (doi)
2017 (11)
- Fritzsch, F.S.O., Blank, L.M., Dusny, C., Schmid, A. (2017):
Miniaturized octupole cytometry for cell type independent trapping and analysis
Microfluid. Nanofluid. 21 (8), art. 130
full text (doi) - Halan, B., Vassilev, I., Lang, K., Schmid, A., Buehler, K. (2017):
Growth of Pseudomonas taiwanensis VLB120ΔC biofilms in the presence of n-butanol
Microb. Biotechnol. 10 (4), 745 - 755
full text (doi) - Hoschek, A., Bühler, B., Schmid, A. (2017):
Overcoming the gas-liquid mass transfer of oxygen by coupling photosynthetic water oxidation with biocatalytic oxyfunctionalization
Angew. Chem.-Int. Edit. 56 (47), 15146 - 15149
full text (doi) - Kadisch, M., Julsing, M.K., Schrewe, M., Jehmlich, N., Scheer, B., von Bergen, M., Schmid, A., Bühler, B. (2017):
Maximization of cell viability rather than biocatalyst activity improves whole-cell ω-oxyfunctionalization performance
Biotechnol. Bioeng. 114 (4), 874 - 884
full text (doi) - Kadisch, M., Schmid, A., Bühler, B. (2017):
Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing
J. Ind. Microbiol. Biotechnol. 44 (3), 339 - 351
full text (doi) - Kadisch, M., Willrodt, C., Hillen, M., Bühler, B., Schmid, A. (2017):
Maximizing the stability of metabolic engineering-derived whole-cell biocatalysts
Biotechnol. J. 12 (8), art. 1600170
full text (doi) - Rosenthal, K., Oehling, V., Dusny, C., Schmid, A. (2017):
Beyond the bulk: disclosing the life of single microbial cells
FEMS Microbiol. Rev. 41 (6), 751 - 780
full text (doi) - Schmutzler, K., Kupitz, K., Schmid, A., Buehler, K. (2017):
Hyperadherence of Pseudomonas taiwanensis VLB120ΔC increases productivity of (S)-styrene oxide formation
Microb. Biotechnol. 10 (4), 735 - 744
full text (doi) - Theodosiou, E., Breisch, M., Julsing, M.K., Falcioni, F., Bühler, B., Schmid, A. (2017):
An artificial TCA cycle selects for efficient α-ketoglutarate dependent hydroxylase catalysis in engineered Escherichia coli
Biotechnol. Bioeng. 114 (7), 1511 - 1520
full text (doi) - Volmer, J., Schmid, A., Bühler, B. (2017):
The application of constitutively solvent-tolerant P. taiwanensis VLB120ΔCΔttgV for stereospecific epoxidation of toxic styrene alleviates carrier solvent use
Biotechnol. J. 12 (7), art. 1600558
full text (doi) - Willrodt, C., Halan, B., Karthaus, L., Rehdorf, J., Julsing, M.K., Buehler, K., Schmid, A. (2017):
Continuous multistep synthesis of perillic acid from limonene by catalytic biofilms under segmented flow
Biotechnol. Bioeng. 114 (2), 281 - 290
full text (doi)
2016 (10)
- Dusny, C., Schmid, A. (2016):
The MOX promoter in Hansenula polymorpha is ultrasensitive to glucose-mediated carbon catabolite repression
FEMS Yeast Res. 16 (6), fow067
full text (doi) - Dusny, C., Schmid, A. (2016):
Neue Einblicke in die Welt isolierter Mikroben
Biospektrum 22 (7), 708 - 711
full text (doi) - Halan, B., Karande, R., Buehler, K., Schmid, A. (2016):
Catalytic Pseudomonas taiwanensis VLB120ΔC biofilms thrive in a continuous pure styrene generated by multiphasic segmented flow in a capillary microreactor
J. Flow Chem. 6 (1), 39 - 42
full text (doi) - Karande, R., Debor, L., Salamanca, D., Bogdahn, F., Engesser, K.-H., Buehler, K., Schmid, A. (2016):
Continuous cyclohexane oxidation to cyclohexanol using a novel cytochrome P450 monooxygenase from Acidovorax sp. CHX100 in recombinant P. taiwanensis VLB120 biofilms
Biotechnol. Bioeng. 113 (1), 52 - 61
full text (doi) - Karande, R., Schmid, A., Buehler, K. (2016):
Applications of multiphasic microreactors for biocatalytic reactions
Org. Process Res. Dev. 20 (2), 361 - 370
full text (doi) - Ladkau, N., Assmann, M., Schrewe, M., Julsing, M.K., Schmid, A., Bühler, B. (2016):
Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli
Metab. Eng. 36 , 1 - 9
full text (doi) - Lange, K., Schmid, A., Julsing, M.K. (2016):
Δ9-Tetrahydrocannabinolic acid synthase: The application of a plant secondary metabolite enzyme in biocatalytic chemical synthesis
J. Biotechnol. 233 , 42 - 48
full text (doi) - Schmutzler, K., Kracht, O.N., Schmid, A., Buehler, K. (2016):
Trophic regulation of autoaggregation in Pseudomonas taiwanensis VLB120
Appl. Microbiol. Biotechnol. 100 (1), 347 - 360
full text (doi) - Sudarsan, S., Blank, L.M., Dietrich, A., Vielhauer, O., Takors, R., Schmid, A., Reuss, M. (2016):
Dynamics of benzoate metabolism in Pseudomonas putida KT2440
Metab. Eng. Commun. 3 , 97 - 110
full text (doi) - Willrodt, C., Hoschek, A., Bühler, B., Schmid, A., Julsing, M.K. (2016):
Decoupling production from growth by magnesium sulfate limitation boosts de novo limonene production
Biotechnol. Bioeng. 113 (6), 1305 - 1314
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2015 (17)
- Collins, J., Grund, M., Brandenbusch, C., Sadowski, G., Schmid, A., Bühler, B. (2015):
The dynamic influence of cells on the formation of stable emulsions in organic–aqueous biotransformations
J. Ind. Microbiol. Biotechnol. 42 (7), 1011 - 1026
full text (doi) - David, C., Bühler, K., Schmid, A. (2015):
Stabilization of single species Synechocystis biofilms by cultivation under segmented flow
J. Ind. Microbiol. Biotechnol. 42 (7), 1083 - 1089
full text (doi) - Dusny, C., Grünberger, A., Probst, C., Wiechert, W., Kohlheyer, D., Schmid, A. (2015):
Technical bias of microcultivation environments on single-cell physiology
Lab Chip 15 (8), 1822 - 1834
full text (doi) - Dusny, C., Schmid, A. (2015):
Challenging biological limits with microfluidic single cell analysis
Microb. Biotechnol. 8 (1), 23 - 25
full text (doi) - Dusny, C., Schmid, A. (2015):
Microfluidic single-cell analysis links boundary environments and individual microbial phenotypes
Environ. Microbiol. 17 (6), 1839 - 1856
full text (doi) - Köhler, K.A.K., Rühl, J., Blank, L.M., Schmid, A. (2015):
Integration of biocatalyst and process engineering for sustainable and efficient n-butanol production
Eng. Life Sci. 15 (1), 4 - 19
full text (doi) - Lang, K., Buehler, K., Schmid, A. (2015):
Multistep synthesis of (S)-3-hydroxyisobutyric acid from glucose using Pseudomonas taiwanensis VLB120 B83 T7 catalytic biofilms
Adv. Synth. Catal. 357 (8), 1919 - 1927
full text (doi) - Lange, K., Schmid, A., Julsing, M.K. (2015):
Δ9-Tetrahydrocannabinolic acid synthase production in Pichia pastoris enables chemical synthesis of cannabinoids
J. Biotechnol. 211 , 68 - 76
full text (doi) - Lindmeyer, M., Jahn, M., Vorpahl, C., Müller, S., Schmid, A., Bühler, B. (2015):
Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains
Front. Microbiol. 6 , art. 1042
full text (doi) - Lindmeyer, M., Meyer, D., Kuhn, D., Bühler, B., Schmid, A. (2015):
Making variability less variable: matching expression system and host for oxygenase-based biotransformations
J. Ind. Microbiol. Biotechnol. 42 (6), 851 - 866
full text (doi) - Rosenthal, K., Falke, F., Frick, O., Dusny, C., Schmid, A. (2015):
An inert continuous microreactor for the isolation and analysis of a single microbial cell
Micromachines 6 (12), 1836 - 1855
full text (doi) - Salamanca, D., Karande, R., Schmid, A., Dobslaw, D. (2015):
Novel cyclohexane monooxygenase from Acidovorax sp. CHX100
Appl. Microbiol. Biotechnol. 99 (16), 6889 - 6897
full text (doi) - Schmutzler, K., Schmid, A., Buehler, K. (2015):
A three-step method for analysing bacterial biofilm formation under continuous medium flow
Appl. Microbiol. Biotechnol. 99 (14), 6035 - 6047
full text (doi) - Theodosiou, E., Frick, O., Bühler, B., Schmid, A. (2015):
Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation
Microb. Cell. Fact. 14 , art. 108
full text (doi) - Volmer, J., Schmid, A., Bühler, B. (2015):
Guiding bioprocess design by microbial ecology
Curr. Opin. Microbiol. 25 , 25 - 32
full text (doi) - Willrodt, C., Hoschek, A., Bühler, B., Schmid, A., Julsing, M.K. (2015):
Coupling limonene formation and oxyfunctionalization by mixed-culture resting cell fermentation
Biotechnol. Bioeng. 112 (9), 1738 - 1750
full text (doi) - Willrodt, C., Karande, R., Schmid, A., Julsing, M.K. (2015):
Guiding efficient microbial synthesis of non-natural chemicals by physicochemical properties of reactants
Curr. Opin. Biotechnol. 35 , 52 - 62
full text (doi)