Dr. Henning Großkopf

 Postdoc in the group of Functional Genomics

Contact

Department of Molecular Systems Biology
Helmholtz Centre for Environmental Research GmbH - UFZ
Permoserstraße 15 | 04318 Leipzig

Phone: +49 341 235 1078
henning.grosskopf@ufz.de

Henning Großkopf

Projects


Investigation of glycosaminoglycans-protein interactions

Due to the demographic change, a considerable increase of bone defects and chronic wounds can be observed. The Transregio 67 research program of the German Research Foundation (DFG) aims for the development of novel biomaterials that support the healing capacities in skin and bone defects. As components of the physiological extracellular matrix, glycosaminoglycans (GAG) are in the focus of this research.
In this project, cellular adaptions to GAG-treatment in terms of altered protein expression or activity of specific signaling pathways are investigated utilizing mass spectrometry-based, quantitative proteomics, and phosphoproteomics. A special focus is on the identification of novel GAG-interacting proteins by means of affinity-purification MS (AP-MS).

The impact of pollutants on ubiquitin-mediated signaling processes of the aryl hydrocarbon receptor in macrophages

The aryl hydrocarbon receptor (AhR) is activated by endogenous ligands, as well as, pollutants, e.g. dioxins and polycyclic aromatic hydrocarbons from combustion processes. The formed receptor-ligand complex translocates to the nucleus and stimulates the expression of target genes. Among these are enzymes which are involved in the metabolisation of xenobiotics, including proteins of the cytochrome P450-family. In addition, the AhR acts as a substrate recognition subunit of Cullin-RING ubiquitin ligases. The complex ubiquitinates target proteins specifically and thus initiates their degradation.
In the course of immunoreactions, the AhR has a regulatory role. Thereby it provides either pro- or anti-inflammatory signals, depending on the specific ligand and cell type. On the one hand, AhR interaction partners in macrophages are investigated by means of affinity-purification MS (AP-MS), in this project. On the other hand, AhR-dependent, ubiquitin-mediated signaling processes are investigated utilizing global ubiquitome and proteome approaches.


Curriculum Vitae


since 2017

Postdoctoral researcher in the Functional Genomics group at the Department of Molecular Systems Biology at the Helmholtz Centre for Environmental Research - UFZ

2013 - 2017

PhD student at the Helmholtz Center for Infection Research (HZI) in the Cellular Proteomics research group
“The role of ubiquitination during B cell proliferation induced by the
Epstein-Barr virus”

2010 - 2012

Master studies, Biochemistry
Hannover Medical School

2007 - 2010

Bachelor studies, Biochemistry
Leibniz University Hannover


Publications


Tsikas, D.; Böhmer, A.; Großkopf, H.; Beckmann, B.; Dreißigacker, U.; Jordan, J.; Massen, N. (2012)
Clinical-chemistry laboratory relevant hemolysis is unlikely to compromise human plasma concentration of free asymmetric dimethylarginine (ADMA).
Clinical Biochemistry.

Großkopf, H.; Böhmer, A.; Tsikas, D. (2012)
Letter to the editor: “Role of the human erythrocyte in generation and storage of asymmetric dimethylarginine.”
American journal of physiology. Heart and circulatory physiology.

Böhmer, A.; Großkopf, H.; Jordan, J.; Tsikas, D. (2012)
Human hemoglobin does not contain asymmetric dimethylarginine (ADMA).
Nitric Oxide.