Dr. Henning Großkopf
Postdoc in the group of Functional Genomics
Contact
Department of Molecular Systems Biology
Helmholtz Centre for Environmental Research GmbH - UFZ
Permoserstraße 15 | 04318 Leipzig
Phone: +49 341 235 1354
henning.grosskopf@ufz.de

Projects
Investigation of glycosaminoglycans-protein interactions
Due to the demographic change, a considerable increase of bone defects and chronic wounds can be observed. The Transregio 67 research program of the German Research Foundation (DFG) aims for the development of novel biomaterials that support the healing capacities in skin and bone defects. As components of the physiological extracellular matrix, glycosaminoglycans (GAG) are in the focus of this research.
In this project, cellular adaptions to GAG-treatment in terms of altered protein expression or activity of specific signaling pathways are investigated utilizing mass spectrometry-based, quantitative proteomics, and phosphoproteomics. A special focus is on the identification of novel GAG-interacting proteins by means of affinity-purification MS (AP-MS).
The impact of pollutants on ubiquitin-mediated signaling processes of the aryl hydrocarbon receptor in macrophages
The aryl hydrocarbon receptor (AhR) is activated by endogenous ligands, as well as, pollutants, e.g. dioxins and polycyclic aromatic hydrocarbons from combustion processes. The formed receptor-ligand complex translocates to the nucleus and stimulates the expression of target genes. Among these are enzymes which are involved in the metabolisation of xenobiotics, including proteins of the cytochrome P450-family. In addition, the AhR acts as a substrate recognition subunit of Cullin-RING ubiquitin ligases. The complex ubiquitinates target proteins specifically and thus initiates their degradation.
In the course of immunoreactions, the AhR has a regulatory role. Thereby it provides either pro- or anti-inflammatory signals, depending on the specific ligand and cell type. On the one hand, AhR interaction partners in macrophages are investigated by means of affinity-purification MS (AP-MS), in this project. On the other hand, AhR-dependent, ubiquitin-mediated signaling processes are investigated utilizing global ubiquitome and proteome approaches.
Curriculum Vitae
since 2017
Postdoctoral researcher in the Functional Genomics group at the Department of Molecular Systems Biology at the Helmholtz Centre for Environmental Research - UFZ
2013 - 2017
PhD student at the Helmholtz Center for Infection Research (HZI) in the Cellular Proteomics research group
“The role of ubiquitination during B cell proliferation induced by the
Epstein-Barr virus”
2010 - 2012
Master studies, Biochemistry
Hannover Medical School
2007 - 2010
Bachelor studies, Biochemistry
Leibniz University Hannover
Publications
Großkopf, H.; Walter, K.; Karkossa, I.; von Bergen, M.; Schubert, K. (2021)
Non-Genomic AhR-Signaling Modulates the Immune Response in Endotoxin-Activated Macrophages After Activation by the Environmental Stressor BaP.
Frontiers in Immunology.
doi: 10.3389/fimmu.2021.620270
Wang, Z.; Karkossa, I.; Großkopf, H.; Rolle-Kampczyk, U.; Hackermüller, J.; von Bergen, M.; Schubert, K. (2020)
Comparison of quantitation methods in proteomics to define relevant toxicological information on AhR activation of HepG2 cells by BaP.
Toxicology.
doi: 10.1016/j.tox.2020.152652
Tsikas, D.; Böhmer, A.; Großkopf, H.; Beckmann, B.; Dreißigacker, U.; Jordan, J.; Massen, N. (2012)
Clinical-chemistry laboratory relevant hemolysis is unlikely to compromise human plasma concentration of free asymmetric dimethylarginine (ADMA).
Clinical Biochemistry.
doi: 10.1016/j.clinbiochem.2012.03.020
Großkopf, H.; Böhmer, A.; Tsikas, D. (2012)
Letter to the editor: “Role of the human erythrocyte in generation and storage of asymmetric dimethylarginine.”
American journal of physiology. Heart and circulatory physiology.
doi: 10.1152/ajpheart.00449.2012
Böhmer, A.; Großkopf, H.; Jordan, J.; Tsikas, D. (2012)
Human hemoglobin does not contain asymmetric dimethylarginine (ADMA).
Nitric Oxide.
doi: 10.1016/j.niox.2012.03.013