Core facilities

Laser Scanning Microscopes

Instrument 1

- combined confocal and 2-photon laser scanning microscope
- TCS SP1 with upright and inverted microscope
- visible laser lines at 458, 476, 488, 514, 561, 633 nm
- 4 detector channels (PMT's)
- pulsed 2-photon Titanium Sapphire laser (760-900 nm)
- Fluorescence Lifetime Imaging Microscopy (FLIM) setup

Instrument 2

- Confocal laser scanning microscope
- TCS SP5X AOBS with upright microscope
- tandem scanner for high resolution and fast imaging
- super continuum light source with laser lines from 470-670 nm
- 5 detector channels (PMT's)
- pulsed laser diodes (405, 470, 640 nm)
- Fluorescence Lifetime Imaging Microscopy (FLIM) setup
- Fluorescence Correlation Spectroscopy (FCS) setup

Instrument 3

- GSD widefield nanoscope (inverted)
- based on ground state depletion with individual molecule return
- laser lines at 488, 532 and 642 nm plus 405 nm
- detector iXon3


Digital Image Analysis

Computer Hardware

- HP Z800 graphic workstation for visualisation and deconvolution
- PC graphic workstation for quantification
- various colour print options including poster print

Available Software

- Imaris (visualisation)
- Huygens (deconvolution)
- ImageJ (NIH) with self written special plugins (quantification)
- Microstat (watershed segmentation, quantification)
- Phlip (quantification, colocalisation)
- ConAn (object recognition, quantification, structural parameters)