Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1111/j.1574-6941.2000.tb00684.x |
| Title (Primary) | Three types of phenol and p-cresol catabolism in phenol- and p-cresol-degrading bacteria isolated from river water continuously polluted with phenolic compounds |
| Author | Heinaru, E.; Truu, J.; Stottmeister, U.; Heinaru, A. |
| Source Titel | FEMS Microbiology Ecology |
| Year | 2000 |
| Department | UBT_alt; SAN |
| Volume | 31 |
| Issue | 3 |
| Page From | 195 |
| Page To | 205 |
| Language | englisch |
| Keywords | Phenol-degrader; p-Cresol-degrader; Catechol 1,2-dioxygenase; Catechol 2,3-dioxygenase; Protocatechuate 3,4-dioxygenase; xylE; pheB; PCR fingerprint; Pseudomonas |
| Abstract | A total of 39 phenol- and p-cresol-degraders isolated from the river water continuously polluted with phenolic compounds of oil shale leachate were studied. Species identification by BIOLOG GN analysis revealed 21 strains of Pseudomonas fluorescens (4, 8 and 9 of biotypes A, C and G, respectively), 12 of Pseudomonas mendocina, four of Pseudomonas putida biotype A1, one of Pseudomonas corrugata and one of Acinetobacter genospecies 15. Computer-assisted analysis of rep-PCR fingerprints clustered the strains into groups with good concordance with the BIOLOG GN data. Three main catabolic types of degradation of phenol and p-cresol were revealed. Type I, or meta-meta type (15 strains), was characterized by meta cleavage of catechol by catechol 2,3-dioxygenase (C23O) during the growth on phenol and p-cresol. These strains carried C23O genes which gave PCR products with specific xylE-gene primers. Type II, or ortho-ortho type (13 strains), was characterized by the degradation of phenol through ortho fission of catechol by catechol 1,2-dioxygenase (C12O) and p-cresol via ortho cleavage of protocatechuic acid by protocatechuate 3,4-dioxygenase (PC34O). These strains carried phenol monooxygenase gene which gave PCR products with pheA-gene primers. Type III, or meta-ortho type (11 strains), was characterized by the degradation of phenol by C23O and p-cresol via the protocatechuate ortho pathway by the induction of PC34O and this carried C23O genes which gave PCR products with C23O-gene primers, but not with specific xylE-gene primers. In type III strains phenol also induced the p-cresol protocatechuate pathway, as revealed by the induction of p-cresol methylhydroxylase. These results demonstrate multiplicity of catabolic types of degradation of phenol and p-cresol and the existence of characteristic assemblages of species and specific genotypes among the strains isolated from the polluted river water. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=7223 |
| Heinaru, E., Truu, J., Stottmeister, U., Heinaru, A. (2000): Three types of phenol and p-cresol catabolism in phenol- and p-cresol-degrading bacteria isolated from river water continuously polluted with phenolic compounds FEMS Microbiol. Ecol. 31 (3), 195 - 205 10.1111/j.1574-6941.2000.tb00684.x |
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