Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1002/etc.5620191015 |
| Document | Shareable Link |
| Title (Primary) | Fixed-effect-level toxicity equivalents - a suitable parameter for assessing ethoxyresorufin-o-deethylase induction potency in complex environmental samples |
| Author | Brack, W.; Segner, H.; Möder, M.; Schüürmann, G. |
| Source Titel | Environmental Toxicology and Chemistry |
| Year | 2000 |
| Department | OEC; ANA; COE |
| Volume | 19 |
| Issue | 10 |
| Page From | 2493 |
| Page To | 2501 |
| Language | englisch |
| Keywords | Ethoxyresorufin- O-deethylase; Toxicity euivalent assessment; Sediment extract; Bioassay-directed fractionation; Confirmation |
| Abstract | Within the scope of bioassay-directed identification of dioxin-like toxicants in complex environmental samples, EC50-based and fixed-effect-level-based 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxicity equivalents (TEQs) were compared to assess 7-ethoxyresorufin-O-deethylase (EROD) inducing potency of sediment fractions using the rainbow trout liver cell line RTL-W1 as bioassay system. Toxicity equivalents on the basis of fixed effect levels are suggested in order to minimize interpretation problems due to the superposition of enzyme-inducing and enzyme-inhibiting effects. Bioassay-directed fractionation of a contaminated sediment extract in the industrial region of Bitterfeld (Germany) based on fixed-effect-level TEQs indicated high dioxin-like activity in the lipophilic sediment fractions containing the prototypic arylhydrocarbon receptor (AhR) agonists polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs). However, only a small part of the EROD induction could be attributed to the PCBs and PAHs that were analyzed. Significant EROD induction occurred also with some of the more polar fractions. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=7024 |
| Brack, W., Segner, H., Möder, M., Schüürmann, G. (2000): Fixed-effect-level toxicity equivalents - a suitable parameter for assessing ethoxyresorufin-o-deethylase induction potency in complex environmental samples Environ. Toxicol. Chem. 19 (10), 2493 - 2501 10.1002/etc.5620191015 |
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