Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.plaphy.2009.02.003
Title (Primary) Effect of trifluoroacetate, a persistent degradation product of fluorinated hydrocarbons, on Phaseolus vulgaris and Zea mays
Author Smit, M.F.; van Heerden, P.D.R.; Pienaar, J.J.; Weissflog, L.; Strasser, R.J.; Krüger, G.H.J.
Source Titel Plant Physiology and Biochemistry
Year 2009
Department ANA
Volume 47
Issue 7
Page From 623
Page To 634
Language englisch
Keywords Phaseolus vulgaris; Zea mays; Trifluoroacetate; Trichloroacetate; Photosynthetic gas exchange; Mesophyll limitation; Stomatal limitation; Photosynthetic electron transport; Chlorophyll a fluorescence; JIP-test; C3 and C4 crop plants; Rubisco activity
Abstract The aim of this study was to quantify the effect of the pollutant, trifluoroacetate (TFA), on growth and photosynthesis of Phaseolus vulgaris (C-3) and Zea mays (C-4) in order to elucidate the physiological and biochemical basis of its inhibitory action. In whole plant studies, photosynthetic gas exchange, fast phase fluorescence kinetics and Rubisco activity were measured in parallel over a 14-day period in plants cultivated in a water culture system with NaTFA added at concentrations ranging from 0.625 to 160 mg l(-1). Although initial stimulation of some photosynthetic parameters was observed at low TEA concentrations early on in the experiment, marked inhibition occurred at higher concentrations. In general Z mays was affected more severely than P. vulgaris showing a large TFA-induced decrease in both apparent carboxylation efficiency (ACE) and in vitro Rubisco(ribulose-1,5-bisphosphate carboxylase/oxygenase: EC 4.1.1.39) activity. Analysis of photosynthetic gas exchange revealed that besides constraints on mesophyll processes suchas Rubisco activity, stomatal limitation also increased with increasing TEA concentration, especially in P vulgaris. In depth analysis of the fast phase fluorescence transients pointed at TFA-induced uncoupling of the oxygen evolving complex (OEC) and inhibition of electron transport beyond Q, including possible constraints on the reduction of end electron acceptors of photosystem I.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=625
Smit, M.F., van Heerden, P.D.R., Pienaar, J.J., Weissflog, L., Strasser, R.J., Krüger, G.H.J. (2009):
Effect of trifluoroacetate, a persistent degradation product of fluorinated hydrocarbons, on Phaseolus vulgaris and Zea mays
Plant Physiol. Biochem. 47 (7), 623 - 634 10.1016/j.plaphy.2009.02.003