Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.jchromb.2006.04.048 |
| Title (Primary) | Comparison of ELISA and capillary electrophoresis with laser-induced fluorescence detection in the analysis of Ochratoxin A in low volumes of human blood serum |
| Author | Köller, G.; Wichmann, G.; Rolle-Kampczyk, U.; Popp, P.; Herbarth, O. |
| Source Titel | Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences |
| Year | 2006 |
| Department | ANA; IMMU; EXPOEPID |
| Volume | 840 |
| Issue | 2 |
| Page From | 94 |
| Page To | 98 |
| Language | englisch |
| Keywords | Capillary electrophoresis; CE-LIF human blood serum; Low sample volume; Ochratoxin A; ELISA |
| Abstract | In this paper the determination of Ochratoxin A (OTA) in low volumes of human blood serum by enzyme-linked immunosorbent assay (ELISA) is compared with an appropriate capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) method. In order to use ELISA for high-throughput analysis in epidemiological studies no sample cleanup was performed. Both methods showed a limit of detection (LOD) of 0.5 ng/mL. Comparing the precisions of both methods, the data show that the quantified concentrations in ELISA are higher than the corresponding concentrations in the CE-LIF method. Using a matrix calibration curve instead of a standard calibration curve the reproducibilities of both methods are comparable. No additional matrix effect could be observed by adding phenylalanine as probable matrix compound to the serum. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2793 |
| Köller, G., Wichmann, G., Rolle-Kampczyk, U., Popp, P., Herbarth, O. (2006): Comparison of ELISA and capillary electrophoresis with laser-induced fluorescence detection in the analysis of Ochratoxin A in low volumes of human blood serum J. Chromatogr. B 840 (2), 94 - 98 10.1016/j.jchromb.2006.04.048 |
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