Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1093/femsec/fiz029
Title (Primary) Investigation of architecture development and phosphate distribution in Chlorella biofilm by complementary microscopy techniques
Author Moreno Osorio, J.H.; Benettoni, P.; Schmidt, M.; Stryhanyuk, H.; Schmitt-Jansen, M.; Pinto, G.; Pollio, A.; Frunzo, L.; Lens, P.N.L.; Richnow, H.H.; Esposito, G.; Musat, N.
Source Titel FEMS Microbiology Ecology
Year 2019
Department ISOBIO; BIOTOX
Volume 95
Issue 4
Page From fiz029
Language englisch
Supplements https://academic.oup.com/femsec/article/95/4/fiz029/5372415#supplementary-data
Keywords Chlorella species; phosphorous distribution; chemical imaging; multimodal imaging; biofilm development; HIM; ToF-SIMS
UFZ wide themes ProVIS;
Abstract

Microalgae biofilms may play an important role in the mitigation and prevention of eutrophication caused by domestic, agricultural and industrial wastewater effluents. Despite their potential, the biofilm development and role in nutrient removal are not well understood. Its clarification requires comprehensive studies of the complex three-dimensional architecture of the biofilm. In this study, we established a multimodal imaging approach to provide key information regarding architecture development and nutrient distribution in the biofilm of two green algae organisms: Chlorella pyrenoidosa and Chlorella vulgaris. Helium ion microscopy (HIM), scanning electron microscopy coupled with energy dispersive X-ray analysis (SEM-EDX) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) were employed for i) elucidation of spatial arrangement, ii) elemental mapping and iii) 3D chemical imaging of the biofilm. The fine structure of the algal biofilm was resolved by HIM, the evidence of the accumulation of phosphate in hot spots was provided by SEM-EDX and the localization of phosphate oxides granules throughout the whole sample was clarified by ToF-SIMS. The reported results shed light on the phosphorus distribution during Chlorella's biofilm formation and highlight the potential of such correlative approach to solve fundamental question in algal biotechnology research.

Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21741
Moreno Osorio, J.H., Benettoni, P., Schmidt, M., Stryhanyuk, H., Schmitt-Jansen, M., Pinto, G., Pollio, A., Frunzo, L., Lens, P.N.L., Richnow, H.H., Esposito, G., Musat, N. (2019):
Investigation of architecture development and phosphate distribution in Chlorella biofilm by complementary microscopy techniques
FEMS Microbiol. Ecol. 95 (4), fiz029 10.1093/femsec/fiz029