Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1007/s00248-017-1100-1
Document Shareable Link
Title (Primary) Anaerobic benzene mineralization by nitrate-reducing and sulfate-reducing microbial consortia enriched from the same site: comparison of community composition and degradation characteristics
Author Keller, A.H.; Kleinsteuber, S. ORCID logo ; Vogt, C.
Source Titel Microbial Ecology
Year 2018
Department ISOBIO; UMB
Volume 75
Issue 4
Page From 941
Page To 953
Language englisch
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs00248-017-1100-1/MediaObjects/248_2017_1100_MOESM1_ESM.docx
https://static-content.springer.com/esm/art%3A10.1007%2Fs00248-017-1100-1/MediaObjects/248_2017_1100_MOESM2_ESM.xlsx
Keywords Contaminated aquifer; Anaerobic benzene degradation; Community shift; Compound-specific stable isotope analysis
UFZ wide themes RU3;
Abstract Benzene mineralization under nitrate-reducing conditions was successfully established in an on-site reactor continuously fed with nitrate and sulfidic, benzene-containing groundwater extracted from a contaminated aquifer. Filling material from the reactor columns was used to set up anoxic enrichment cultures in mineral medium with benzene as electron donor and sole organic carbon source and nitrate as electron acceptor. Benzene degradation characteristics and community composition under nitrate-reducing conditions were monitored and compared to those of a well-investigated benzene-mineralizing consortium enriched from the same column system under sulfate-reducing conditions. The nitrate-reducing cultures degraded benzene at a rate of 10.1 ± 1.7 μM d−1, accompanied by simultaneous reduction of nitrate to nitrite. The previously studied sulfate-reducing culture degraded benzene at similar rates. Carbon and hydrogen stable isotope enrichment factors determined for nitrate-dependent benzene degradation differed significantly from those of the sulfate-reducing culture (ΛH/C nitrate = 12 ± 3 compared to ΛH/C sulfate = 28 ± 3), indicating different benzene activation mechanisms under the two conditions. The nitrate-reducing community was mainly composed of Betaproteobacteria, Ignavibacteria, and Anaerolineae. Azoarcus and a phylotype related to clone Dok59 (Rhodocyclaceae) were the dominant genera, indicating an involvement in nitrate-dependent benzene degradation. The primary benzene degrader of the sulfate-reducing consortium, a phylotype belonging to the Peptococcaceae, was absent in the nitrate-reducing consortium.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19518
Keller, A.H., Kleinsteuber, S., Vogt, C. (2018):
Anaerobic benzene mineralization by nitrate-reducing and sulfate-reducing microbial consortia enriched
from the same site: comparison of community composition and degradation characteristics
Microb. Ecol. 75 (4), 941 - 953 10.1007/s00248-017-1100-1