Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1002/eji.201646297
Title (Primary) High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition
Author Zimmermann, J.; Hübschmann, T.; Schattenberg, F.; Schumann, J.; Durek, P.; Riedel, R.; Friedrich, M.; Glauben, R.; Siegmund, B.; Radbruch, A.; Müller, S.; Chang, H.-D.
Source Titel European Journal of Immunology
Year 2016
Department UMB
Volume 46
Issue 5
Page From 1300
Page To 1303
Language englisch
Keywords Flow cytometry; IBD; Microbiota; Single-cell analysis; T-cell transfer colitis
UFZ wide themes RU3;
Abstract Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17808
Zimmermann, J., Hübschmann, T., Schattenberg, F., Schumann, J., Durek, P., Riedel, R., Friedrich, M., Glauben, R., Siegmund, B., Radbruch, A., Müller, S., Chang, H.-D. (2016):
High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition
Eur. J. Immunol. 46 (5), 1300 - 1303 10.1002/eji.201646297