Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1111/1574-6941.12097
Title (Primary) Alkane-degrading bacteria at the soil–litter interface: comparing isolates with T-RFLP-based community profiles
Author Giebler, J.; Wick, L.Y.; Chatzinotas, A.; Harms, H.
Source Titel FEMS Microbiology Ecology
Year 2013
Department UMB
Volume 86
Issue 1
Page From 45
Page To 58
Language englisch
Keywords soil alkane degradation potential;cultivation;growth matrix-based enrichment;alkB T-RFLP;seed bank;rare biosphere
UFZ wide themes ru3
Abstract

Alkane-degrading bacteria were isolated from uncontaminated soil microcosms, which had been incubated with maize litter as natural alkane source. The isolates served to understand spatio-temporal community changes at the soil–litter interface, which had been detected using alkB as a functional marker gene for bacterial alkane degraders. To obtain a large spectrum of isolates, liquid subcultivation was combined with a matrix-assisted enrichment (Teflon membranes, litter). Elevated cell numbers of alkane degraders were detected by most probable number counting indicating enhanced alkane degradation potential in soil in response to litter treatment. Partial 16S rRNA gene sequencing of 395 isolates revealed forty different phylogenetic groups [operational taxonomic units (OTUs)] and spatio-temporal shifts in community composition. Ten OTUs comprised so far unknown alkane degraders, and five OTUs represented putative new bacterial genera. The combination of enrichment methods yielded a higher diversity of isolates than liquid subcultivation alone. Comparison of 16S rRNA gene T-RFLP profiles indicated that many alkane degraders present in the enrichments were not detectable in the DNA extracts from soil microcosms. These possibly rare specialists might represent a seed bank for the alkane degradation capacity in uncontaminated soil. This relevant ecosystem function can be fostered by the formation of the soil–litter interface.

Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14114
Giebler, J., Wick, L.Y., Chatzinotas, A., Harms, H. (2013):
Alkane-degrading bacteria at the soil–litter interface: comparing isolates with T-RFLP-based community profiles
FEMS Microbiol. Ecol. 86 (1), 45 - 58 10.1111/1574-6941.12097