Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.limno.2009.10.003
Title (Primary) Microbial iron reduction during passive in situ remediation of an acidic mine pit lake mesocosm
Author Wendt-Potthoff, K. ORCID logo ; Bozau, E.; Frömmichen, R.; Meier, J.; Koschorreck, M.
Source Titel Limnologica
Year 2010
Department SEEFO
Volume 40
Issue 2
Page From 175
Page To 181
Language englisch
Keywords Acidic pit lake; Microbial Fe(III) reduction; Iron; Microbial sulfate reduction; Passive treatment; Bacteria; Sediment; Lusatia
Abstract Ferric iron reduction was studied in a pilot-scale enclosure experiment for passive biological remediation of an acidic mine pit lake in Lusatia, Germany. The metabolic properties of prokaryotes involved in Fe(III) reduction may be important for the outcome of biological remediation, as chemolithotrophic Fe(III) reduction can counteract the desired pH increase, but heterotrophic Fe(III) reduction will provide the necessary Fe(II) for precipitation of sulfide minerals following sulfate reduction. Therefore, vertical profiles of sediment parameters related to iron and sulfur cycling were determined in conjunction with viable counts of different ferric iron-reducing micro-organisms using selective media. Findings were compared to an untreated reference site. The addition of organic matter stimulated ferric iron reduction and sulfate reduction in the enclosure and led to elevated pH and accumulations of ferrous iron and reduced sulfur compounds. Numbers of neutrophilic heterotrophic Fe(III) reducers increased during treatment, those of acidophilic heterotrophic Fe(III) reducers remained similar, and those of acidophilic chemolithotrophic Fe(III) reducers decreased. Zones of ferric iron-reducing activity corresponded well with microbial depth profiles; however, viable counts of neutrophilic or acid-tolerant Fe(III) reducers must have been underestimated based on the corresponding observed activity levels. Ferric iron reduction by chemolithotrophic acidophiles seemed to be of minor importance, so a lowering of pH values due to Fe(III) reducing activity is unlikely.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10652
Wendt-Potthoff, K., Bozau, E., Frömmichen, R., Meier, J., Koschorreck, M. (2010):
Microbial iron reduction during passive in situ remediation of an acidic mine pit lake mesocosm
Limnologica 40 (2), 175 - 181 10.1016/j.limno.2009.10.003