Dr. Isabel Karkossa
(Née Kratochvil)
Postdoc in der Functional Genomics Gruppe
Kontakt
Department Molekulare Systembiologie (MOLSYB)
Helmholtz-Zentrum für Umweltforschung - UFZ
Permoser Str. 15
04318 Leipzig
Tel: +49 341 235 1354
isabel.karkossa@ufz.de
ResearchGate
@IKarkossa
Forschungsschwerpunkte
- Systembiologische Analyse der modes of action von Schadstoffen in Immunzellen
Proteomics
Lebenslauf
2019 - heute
Postdoc
Helmholtz-Zentrum für Umweltforschung, Leipzig
2016 - 2019
Doktorarbeit
Helmholtz-Zentrum für Umweltforschung, Leipzig
Proteomics zur Bewertung von Nanomaterialien entsprechend ihrer Wirkung
2015
Forschungsaufenthalt
Vanderbilt University, Nashville, TN, USA
Untersuchung von Biomolekülen mittels Ionenmobilitäts-Massenspektrometrie
2014 - 2016
Masterstudium Biochemie
Universität Leipzig, Schwerpunkt Biomedizin
Untersuchung von Interaktionen des PPARγ mit Phthalaten mittels HDX-MS
2011 - 2014
Bachelorstudium Biochemie
Martin-Luther-Universität Halle-Wittenberg
Evaluierung eines MS/MS-spaltbaren Crosslinkers zur 3D-Strukturvorhersage von Proteinen
Publikationen
2023 (11)
- Aldehoff, A.S., Karkossa, I., Engelmann, B., Rolle-Kampczyk, U., von Bergen, M., Schubert, K. (2023):
Exploring the regulatory effects of lysine acetylation and phosphorylation in adipocyte differentiation and their exposure to emerging plastic additives
Toxicol. Lett. 384 (Suppl. 1), S133 - S133
Volltext (DOI) - Bifari, F., Dolci, S., Mannino, L., Campanelli, A., Bottani, E., Karkossa, I., Setten, E., Gianoli, S., Di Chio, M., Ciarpella, F., Busetto, G., Pezzotta, A., Castagna, A., Pruonto, G., Binaschi, L., Amenta, A., Scupoli, M., Cavallini, C., Boschi, F., Doherty, A., Malpeli, G., Rossi, E., Martinelli, N., Ferretti, S., Malik, Z., Sandri, M., Pistocchi, A., Schubert, K., Endrizzi, M., Bossolasco, P., Fumagalli, G., Locati, M., Decimo, I. (2023):
Adoptive transfer of tumor-educated macrophages promote neural tissue regeneration and motor recovery following severe spinal cord injury
Cytotherapy 25 (6, Suppl.), E8 - E9
Volltext (DOI) - Gurbuz, B., Guldiken, N., Reuken, P., Fu, L., Remih, K., Preisinger, C., Brůha, R., Leníček, M., Petrtýl, J., Reissing, J., Aly, M., Fromme, M., Zhou, B., Karkossa, I., Schubert, K., von Bergen, M., Stallmach, A., Bruns, T., Strnad, P. (2023):
Biomarkers of hepatocellular synthesis in patients with decompensated cirrhosis
Hepatol. Int. 17 (3), 698 - 708
Volltext (DOI) - Höper, T., Karkossa, I., Dumit, V.I., von Bergen, M., Schubert, K., Haase, A. (2023):
A comparative proteomics analysis of four contact allergens in THP-1 cells shows distinct alterations in key metabolic pathways
Toxicol. Appl. Pharmacol. 475 , art. 116650
Volltext (DOI) - Karkossa, I., Fürst, S., Großkopf, H., von Bergen, M., Schubert, K. (2023):
Oxidation is an underappreciated post-translational modification in the regulation of immune responses associated with changes in phosphorylation
Front. Immunol. 14 , art. 1244431
Volltext (DOI) - Lipaeva, P., Karkossa, I., Bedulina, D., Schubert, K., Luckenbach, T. (2023):
Cold-adapted amphipod species upon heat stress: Proteomic responses and their correlation with transcriptomic responses
Comp. Biochem. Physiol. D-Genomics Proteomics 45 , art. 101048
Volltext (DOI) - Remih, K., Hufnagel, F., Durkalski-Mauldin, V., Lee, W.M., Su, C., Rule, J., Krieg, L., Karkossa, I., Schubert, K., von Bergen, M., Fontana, R., Strnad, P. (2023):
Serum proteomics in American adults with acute liver failure
J. Hepatol. 78 (Suppl. 1), S136 - S138
Volltext (DOI) - Remih, K., Hufnagel, F.-M., Durkalski-Mauldin, V., Lee, W., Su, Z., Rule, J., Krieg, L., Karkossa, I., Schubert, K., von Bergen, M., Fontana, R., Strnad, P. (2023):
Serum proteomics in adults with Acute Liver Failure
Z. Gastroent. 61 (08), e460 - e461
Volltext (DOI) - Schubert, K., Karkossa, I. (2023):
Effects of high molecular weight phthalates on LPS-stimulated THP-1 macrophages - proteome
PRoteomics IDEntifications Database (PRIDE) 10.6019/PXD043025 - Schubert, K., Karkossa, I. (2023):
Effects of high molecular weight phthalates on LPS-stimulated THP-1 macrophages - redoxome
PRoteomics IDEntifications Database (PRIDE) 10.6019/PXD043139 - Schubert, K., Karkossa, I. (2023):
Effects of high molecular weight phthalates on LPS-stimulated THP-1 macrophages - phosphoproteome
PRoteomics IDEntifications Database (PRIDE) 10.6019/PXD042912
2022 (5)
- Krieg, L., Didt, K., Karkossa, I., Bernhart, S.H., Kehr, S., Subramanian, N., Lindhorst, A., Schaudinn, A., Tabei, S., Keller, M., Stumvoll, M., Dietrich, A., von Bergen, M., Stadler, P.F., Laurencikiene, J., Krüger, M., Blüher, M., Gericke, M., Schubert, K., Kovacs, P., Chakaroun, R., Massier, L. (2022):
Multiomics reveal unique signatures of human epiploic adipose tissue related to systemic insulin resistance
Gut 71 (11), 2179 - 2193
Volltext (DOI) - Lentz, L.S., Stutz, A.J., Meyer, N., Schubert, K., Karkossa, I., von Bergen, M., Zenclussen, A.C., Schumacher, A. (2022):
Human chorionic gonadotropin promotes murine Treg cells and restricts pregnancy-harmful proinflammatory Th17 responses
Front. Immunol. 13 , art. 989247
Volltext (DOI) - Murthy, S., Karkossa, I., Schmidt, C., Hoffmann, A., Hagemann, T., Rothe, K., Seifert, O., Anderegg, U., von Bergen, M., Schubert, K., Rossol, M. (2022):
Danger signal extracellular calcium initiates differentiation of monocytes into SPP1/osteopontin-producing macrophages
Cell Death Dis. 13 , art. 53
Volltext (DOI) - Schaffert, A., Karkossa, I., Ueberham, E., Schlichting, R., Walter, K., Arnold, J., Blüher, M., Heiker, J.T., Lehmann, J., Wabitsch, M., Escher, B.I., von Bergen, M., Schubert, K. (2022):
Di-(2-ethylhexyl) phthalate substitutes accelerate human adipogenesis through PPARγ activation and cause oxidative stress and impaired metabolic homeostasis in mature adipocytes
Environ. Int. 164 , art. 107279
Volltext (DOI) - Schubert, K., Karkossa, I. (2022):
Proteomic characterization of calcium macrophages
PRoteomics IDEntifications Database (PRIDE) 10.6019/PXD026888
2021 (11)
- Großkopf, H., Walter, K., Karkossa, I., von Bergen, M., Schubert, K. (2021):
Non-genomic AhR-signaling modulates the immune response in endotoxin-activated macrophages after activation by the environmental stressor BaP
Front. Immunol. 12 , art. 620270
Volltext (DOI) - Karkossa, I., Bannuscher, A., Hellack, B., Wohlleben, W., Laloy, J., Stan, M.S., Dinischiotu, A., Wiemann, M., Luch, A., Haase, A., von Bergen, M., Schubert, K. (2021):
Nanomaterials induce different levels of oxidative stress, depending on the used model system: Comparison of in vitro and in vivo effects
Sci. Total Environ. 801 , art. 149538
Volltext (DOI) - Karkossa, I., Bannuscher, A., Hellack, B., Wohlleben, W., Laloy, J., Stan, M.S., Dinischiotu, A., Wiemann, M., Luch, A., Haase, A., von Bergen, M., Schubert, K. (2021):
Omics reveal different oxidative stress levels induced by nanomaterials in vitro and in vivo
Toxicol. Lett. 350 (Suppl.), S125
Volltext (DOI) - Monikh, F.A., Chupani, L., Karkossa, I., Gardian, Z., Arenas-Logo, D., von Bergen, M., Schubert, K., Piackova, V., Zuskova, E., Jiskoot, W., Vijver, M.G., Peijnenburg, W.J.G.M. (2021):
An environmental ecocorona influences the formation and evolution of the biological corona on the surface of single-walled carbon nanotubes
NanoImpact 22 , art. 100315
Volltext (DOI) - Schaffert, A., Arnold, J., Karkossa, I., Blüher, M., von Bergen, M., Schubert, K. (2021):
The emerging plasticizer alternative DINCH and its metabolite MINCH induce oxidative stress and enhance inflammatory responses in human THP-1 macrophages
Cells 10 (9), art. 2367
Volltext (DOI) - Schaffert, A., Krieg, L., Weiner, J., Schlichting, R., Ueberham, E., Karkossa, I., Bauer, M., Landgraf, K., Junge, K.M., Wabitsch, M., Lehmann, J., Escher, B.I., Zenclussen, A.C., Körner, A., Blüher, M., Heiker, J.T., von Bergen, M., Schubert, K. (2021):
Alternatives for the worse: Molecular insights into adverse effects of bisphenol a and substitutes during human adipocyte differentiation
Environ. Int. 156 , art. 106730
Volltext (DOI) - Schaffert, A., Krieg, L., Weinert, J., Schlichting, R., Ueberham, E., Karkossa, I., Bauer, M., Junge, K., Wabitsch, M., Lehmann, J., Escher, B.I., Zenclussen, A.C., Körner, A., Blüher, M., Heiker, J.T., von Bergen, M., Schubert, K. (2021):
Emerging substitutes of BPA cause inflammation-like state and impaired insulin sensitivity in differentiating human adipocytes
Toxicol. Lett. 350 (Suppl.), S130
Volltext (DOI) - Schubert, K., Karkossa, I., Schor, J., Engelmann, B., Steinheuer, L.M., Bruns, T., Rolle-Kampczyk, U., Hackermüller, J., von Bergen, M. (2021):
A multi-omics analysis of mucosal-associated-invariant T cells reveals key drivers of distinct modes of activation
Front. Immunol. 12 , art. 616967
Volltext (DOI) - Streidl, T., Karkossa, I., Segura Muñoz, R.R., Eberl, C., Zaufel, A., Plagge, J., Schmaltz, R., Schubert, K., Basic, M., Schneider, K.M., Afify, M., Trautwein, C., Tolba, R., Stecher, B., Doden, H.L., Ridlon, J.M., Ecker, J., Moustafa, T., von Bergen, M., Ramer-Tait, A.E., Clavel, T. (2021):
The gut bacterium Extibacter muris produces secondary bile acids and influences liver physiology in gnotobiotic mice
Gut Microbes 13 (1), 1 - 21
Volltext (DOI) - Walter, K., Grosskopf, H., Karkossa, I., von Bergen, M., Schubert, K. (2021):
Proteomic characterization of the cellular effects of AhR activation by microbial tryptophan catabolites in endotoxin-activated human macrophages
Int. J. Environ. Res. Public Health 18 (19), 10336
Volltext (DOI) - Wang, Z., Karkossa, I., Großkopf, H., Canzler, S., Rolle-Kampczyk, U., Hackermüller, J., von Bergen, M., Schubert, K. (2021):
Quantitative proteomics reveal mechanistic insights into direct and indirect drug-induced thyroid toxicity in rats
Toxicol. Lett. 350 (Suppl.), S129
Volltext (DOI)
2020 (5)
- Bannuscher, A., Karkossa, I., Buhs, S., Nollau, P., Kettler, K., Balas, M., Dinischiotu, A., Hellack, B., Wiemann, M., Luch, A., von Bergen, M., Haase, A., Schubert, K. (2020):
A multi-omics approach reveals mechanisms of nanomaterial toxicity and structure–activity relationships in alveolar macrophages
Nanotoxicology 14 (2), 181 - 195
Volltext (DOI) - Hsu, M.-J., Karkossa, I., Schäfer, I., Christ, M., Kühne, H., Schubert, K., Rolle-Kampczyk, U.E., Kalkhof, S., Nickel, S., Seibel, P., von Bergen, M., Christ, B. (2020):
Mitochondrial transfer by human mesenchymal stromal cells ameliorates hepatocyte lipid load in a mouse model of NASH
Biomedicines 8 (9), art. 350
Volltext (DOI) - Karkossa, I., Raps, S., von Bergen, M., Schubert, K. (2020):
Systematic review of multi-omics approaches to investigate toxicological effects in macrophages
Int. J. Mol. Sci. 21 (24), art. 9371
Volltext (DOI) - Leppert, B., Strunz, S., Seiwert, B., Schlittenbauer, L., Schlichting, R., Pfeifer, C., Röder, S., Bauer, M., Borte, M., Stangl, G.I., Schöneberg, T., Schulz, A., Karkossa, I., Rolle-Kampczyk, U.E., Thürmann, L., von Bergen, M., Escher, B.I., Junge, K.M., Reemtsma, T., Lehmann, I., Polte, T. (2020):
Maternal paraben exposure triggers childhood overweight development
Nat. Commun. 11 , art. 561
Volltext (DOI) - Wang, Z., Karkossa, I., Großkopf, H., Rolle-Kampczyk, U., Hackermüller, J., von Bergen, M., Schubert, K. (2020):
Comparison of quantitation methods in proteomics to define relevant toxicological information on AhR activation of HepG2 cells by BaP
Toxicology 448 , art. 152652
Volltext (DOI)
2019 (4)
- Karkossa, I., Bannuscher, A., Hellack, B., Bahl, A., Buhs, S., Nollau, P., Luch, A., Schubert, K., von Bergen, M., Haase, A. (2019):
An in-depth multi-omics analysis in RLE-6TN rat alveolar epithelial cells allows for nanomaterial categorization
Part. Fibre Toxicol. 16 (1), art. 38
Volltext (DOI) - Kratochvil, I., Bannuscher, A., Hellack, B., von Bergen, M., Haase, A., Schubert, K. (2019):
Nanomaterials affect oxidative phosphorylation and phagocytosis in alveolar macrophages
Eur. J. Immunol. 49 (Suppl. 1), 232
Volltext (DOI) - Kratochvil, I., Hofmann, T., Rother, S., Schlichting, R., Moretti, R., Scharnweber, D., Hintze, V., Escher, B.I., Meiler, J., Kalkhof, S., von Bergen, M. (2019):
Mono(2‐ethylhexyl) phthalate (MEHP) and mono(2‐ethyl‐5‐oxohexyl) phthalate (MEOHP) but not di(2‐ethylhexyl) phthalate (DEHP) bind productively to the peroxisome proliferator‐activated receptor γ
Rapid Commun. Mass Spectrom. 33 (S1), 75 - 85
Volltext (DOI) - Schubert, K., Schor, J., Kratochvil, I., Hackermüller, J., von Bergen, M. (2019):
Multi-omics profiling of MAIT cells reveals specific patterns of antigen-dependent and -independent activation
Eur. J. Immunol. 49 (Suppl. 1), 141
Volltext (DOI)
2018 (1)
- May, J.C., Jurneczko, E., Stow, S.M., Kratochvil, I., Kalkhof, S., McLean, J.A. (2018):
Conformational landscapes of ubiquitin, cytochrome c, and myoglobin: Uniform field ion mobility measurements in helium and nitrogen drift gas
Int. J. Mass Spectrom. 427 , 79 - 90
Volltext (DOI)
- Ihling, C., Falvo, F., Kratochvil, I., Sinz, A., & Schäfer, M. (2015). Dissociation behavior of a bifunctional tempo-active ester reagent for peptide structure analysis by free radical initiated peptide sequencing (FRIPS) mass spectrometry. J Mass Spectrom, 50(2), 396-406
Poster & Talks
Karkossa, I., Bannuscher, A., Hellack, B., Wohlleben, W., Laloy, J., Stan, M.S., Dinischiotu, A., Wiemann, M., Luch, L., Haase, A., von Bergen, M., Schubert, K. (2021). Omics reveal different oxidative stress levels induced by nanomaterials in vitro and in vivo. Poster presented at the Eurotox, virtual meeting.
Karkossa, I., Bannuscher, A., Hellack, B., Haase, A., Schubert, K., von Bergen, M. (2019). Weighted Gene Correlation Network Analysis of multi-omics data highlights effect-inducing properties and biomarker candidates: An application for nanomaterial grouping. Poster presented at the Young Scientists Fall Meeting of the German Society of Mass Spectrometry, Hünfeld, Germany.
Karkossa, I., Bannuscher, A., Hellack, B., von Bergen, M., Haase, A., Schubert, K. (2019). Nanomaterials affect oxidative phosphorylation and phagocytosis in alveolar macrophages. Poster presented at Joint Meeting of the German Society for Immunology and the Italian Society of Immunology, München, Germany.
Kratochvil, I., Bannuscher, A., Hellack, B., Haase A., Schubert, K., von Bergen, M. (2019). Multi-Omics to Develop Nanomaterial Grouping Strategies for Risk Assessment. Poster presented at Proteomic Forum, Potsdam, Germany.
Kratochvil, I., Bannuscher, A., Kettler, K., Haase A., von Bergen, M., Schubert, K. (2018). Weighted Gene Correlation Network Analysis to develop nanomaterial grouping strategies. Talk at 9th International Conference on Nanotoxicology, Neuss, Germany.
Kratochvil, I., Bannuscher, A., Kettler, K., Haase A., Schubert, K., von Bergen, M. (2018). Weighted Gene Correlation Network Analysis as Grouping Strategy for Nanomaterial Risk Assessment in Alveolar Macrophages. Poster presented at European Mass Spectrometry Conference, Saarbrücken, Germany.
Kratochvil, I., Bannuscher, A., Haase A., Schubert K., von Bergen, M. (2018). Weighted Gene Correlation Network Analysis as Grouping Strategy for Nanomaterial Risk Assessment. Poster presented at Research Festival, Leipzig, Germany.
Kratochvil, I., Hofmann, T., Schlichting R., Escher E., Kalkhof, S., von Bergen, M. (2017). MEHP and MEOHP but not DEHP bind and activate the peroxisome proliferator-activated receptor γ. Poster presented at meeting of German Society of Mass Spectrometry, Kiel, Germany.