Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/S0014-5793(97)00480-8
Titel (primär) Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP
Autor Polte, T.; Oberle, S.; Schröder, H.
Quelle FEBS Letters
Erscheinungsjahr 1997
Department IMMU
Band/Volume 409
Heft 1
Seite von 46
Seite bis 48
Sprache englisch
Keywords Nitric oxide; Endothelial cell; Cyclic GMP; Cytoprotection; Tumor necrosis factor-a
Abstract In cultured endothelial cells, incubation with TNF-a (50 ng/ml) for 48 h markedly reduced viability of endothelial cells. A 6 h preincubation with Sper/NO (0.03-1 mu M) protected endothelial cells in a concentration-dependent manner and increased viability by 63% of control. The NO scavenger PTIO (30 mu M) completely abolished cytoprotection by Sper/NO. A cytoprotective effect comparable to Sper/NO was observed when preincubating the cells with 8-bromo cyclic GMP (1-10 mu M). Moreover, no protection by Sper/NO occurred in the presence of ODQ (0.1 mu M), a selective inhibitor of soluble guanylyl cyclase. Our results demonstrate that NO produces a long-term endothelial protection against cellular injury by TNF-alpha, presumably via a cyclic GMP-dependent pathway. (C) 1997 Federation of European Biochemical Societies
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=9542
Polte, T., Oberle, S., Schröder, H. (1997):
Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP
FEBS Lett. 409 (1), 46 - 48 10.1016/S0014-5793(97)00480-8