Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Zeitschriften |
DOI | 10.1016/S0014-5793(97)00480-8 |
Titel (primär) | Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP |
Autor | Polte, T.; Oberle, S.; Schröder, H. |
Quelle | FEBS Letters |
Erscheinungsjahr | 1997 |
Department | IMMU |
Band/Volume | 409 |
Heft | 1 |
Seite von | 46 |
Seite bis | 48 |
Sprache | englisch |
Keywords | Nitric oxide; Endothelial cell; Cyclic GMP; Cytoprotection; Tumor necrosis factor-a |
Abstract | In cultured endothelial cells, incubation with TNF-a (50 ng/ml) for 48 h markedly reduced viability of endothelial cells. A 6 h preincubation with Sper/NO (0.03-1 mu M) protected endothelial cells in a concentration-dependent manner and increased viability by 63% of control. The NO scavenger PTIO (30 mu M) completely abolished cytoprotection by Sper/NO. A cytoprotective effect comparable to Sper/NO was observed when preincubating the cells with 8-bromo cyclic GMP (1-10 mu M). Moreover, no protection by Sper/NO occurred in the presence of ODQ (0.1 mu M), a selective inhibitor of soluble guanylyl cyclase. Our results demonstrate that NO produces a long-term endothelial protection against cellular injury by TNF-alpha, presumably via a cyclic GMP-dependent pathway. (C) 1997 Federation of European Biochemical Societies |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=9542 |
Polte, T., Oberle, S., Schröder, H. (1997): Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP FEBS Lett. 409 (1), 46 - 48 10.1016/S0014-5793(97)00480-8 |