Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1006/bbrc.1998.9486 |
| Titel (primär) | Cyclic AMP mediates endothelial protection by nitric oxide |
| Autor | Polte, T.; Schröder, H. |
| Quelle | Biochemical and Biophysical Research Communications |
| Erscheinungsjahr | 1998 |
| Department | IMMU |
| Band/Volume | 251 |
| Heft | 2 |
| Seite von | 460 |
| Seite bis | 465 |
| Sprache | englisch |
| Abstract | Incubation with TNF-alpha (50 ng/ml) for 72 hours markedly reduced viability of endothelial cells. A 6-hour preincubation with S-nitroso-N-acetyl-D,L-penicillamine,L-penicillamine (SNAP, 3-100 mu M) protected cells in a concentration-dependent manner and decreased TNF-alpha-mediated toxicity by up to 70%. Cytoprotection by SNAP was completely abolished by the adenylyl cyclase inhibitor 2',5'-dideoxyadenosine and mimicked by 8-bromo cyclic AMP or forskolin. SNAP produced significant increases in cyclic GMP and cyclic AMP, both being abrogated in the presence of the NO scavenger 2-phenyl-4,4,5,5,-tetramethylimidazoline-1-oxyl-3-oxide (PTIO). Moreover, no endothelial protection by SNAP was detected in the presence of the protein kinase A inhibitor KT5720, whereas the protein kinase G inhibitor KT5823 left cytoprotection virtually unaltered. Our results demonstrate a crucial role for cyclic AMP in mediating NO-induced endothelial protection against TNF-alpha, possibly through cyclic GMP-dependent inhibition of cyclic AMP breakdown. NO-dependent endothelial protection mag ultimately result from cyclic AMP-induced up-regulation of antioxidant proteins or down-regulation of cytotoxic processes. (C) 1998 Academic Press |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=8912 |
| Polte, T., Schröder, H. (1998): Cyclic AMP mediates endothelial protection by nitric oxide Biochem. Biophys. Res. Commun. 251 (2), 460 - 465 10.1006/bbrc.1998.9486 |
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