Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1161/01.RES.82.9.1016 |
| Titel (primär) | Aspirin increases ferritin synthesis in endothelial cells - A novel antioxidant pathway |
| Autor | Oberle, S.; Polte, T.; Abate, A.; Podhaisky, H.P.; Schröder, H. |
| Quelle | Circulation Research |
| Erscheinungsjahr | 1998 |
| Department | IMMU |
| Band/Volume | 82 |
| Heft | 9 |
| Seite von | 1016 |
| Seite bis | 1020 |
| Sprache | englisch |
| Keywords | aspirin; ferritin; endothelial cell; gene expression; antioxidant defense mechanism |
| Abstract | Aspirin has recently been shown to increase endothelial resistance to oxidative damage, However, the mechanism underlying aspirin-induced cytoprotection is still unknown. Using cultured cells, the present study investigates the effect of aspirin on the expression of ferritin, a cytoprotective protein that sequesters free cytosolic iron, the main catalyst of oxygen radical formation. In bovine pulmonary artery endothelial cells, aspirin at low antithrombotic concentrations (0.03 to 0.3 mmol/L) induced the synthesis of ferritin protein in a time-and concentration-dependent fashion up to 5-fold over basal levels, whereas ferritin H (heavy chain) mRNA remained unaltered. Aspirin-induced cytoprotection from hydrogen peroxide toxicity was mimicked by exogenous iron-free apoferritin but not iron-loaded ferritin, demonstrating the antioxidant function of newly synthesized ferritin under these conditions. Ferritin induction by aspirin was specific in that other nonsteroidal anti-inflammatory drugs such as salicylic acid, indomethacin, or diclofenac failed to alter ferritin protein levels. Aspirin-induced ferritin synthesis was abrogated in the presence of the iron chelator desferrioxamine, pointing to an interaction of aspirin with iron-responsive activation of ferritin translation. Together, our results suggest induction of ferritin as a novel mechanism by which aspirin may prevent endothelial injury in cardiovascular disease, eg, during atherogenesis |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=8890 |
| Oberle, S., Polte, T., Abate, A., Podhaisky, H.P., Schröder, H. (1998): Aspirin increases ferritin synthesis in endothelial cells - A novel antioxidant pathway Circ. Res. 82 (9), 1016 - 1020 10.1161/01.RES.82.9.1016 |
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