Details zur Publikation
Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.freeradbiomed.2004.04.003
Titel (primär) Involvement of lipid peroxidation and organic peroxides in UVA-induced matrix metalloproteinase-1 expression
Autor Polte, T.; Tyrrell, R.M.
Quelle Free Radical Biology and Medicine
Erscheinungsjahr 2004
Department IMMU
Band/Volume 36
Heft 12
Seite von 1566
Seite bis 1574
Sprache englisch
Keywords UVA; Collagenase; Lipid peroxidation; Skin fibroblasts; Free radicals
Abstract Ultraviolet A (UVA) irradiation causes human skin aging and skin cancer at least partially through the activation of matrix metalloproteinases (MMPs). MMP-1, the interstitial collagenase, is responsible for the degradation of collagen and is involved in tumor progression in human skin. The present study uses human skin fibroblast cells (FEK4) to investigate the involvement of lipid peroxidation and the role of peroxides as possible mediators in MMP-1 activation by UVA. Preincubation with the antioxidants butylated hydroxytoluene and Trolox reduced UVA-dependent MMP-1 upregulation, suggesting that peroxidation of membrane lipids is involved. Blocking the iron-driven generation of lipid peroxides and hydroxyl radicals by different iron chelators led to a decrease in UVA-induced MMP-1 mRNA accumulation. Moreover, modulation of glutathione peroxidase activity by use of the specific inhibitor mercaptosuccinate (MS) or by the depletion of glutathione (using buthionine-S, R-sulfoximine, BSO), enhanced the UVA-dependent MMP-1 response. Finally, UVA irradiation generated a significant increase in intracellular peroxide levels which is augmented by pretreatment of the cells with BSO or MS. Our results demonstrate that lipid peroxidation and the production of peroxides are important events in the signalling pathway of MMP-1 activation by UVA. (C) 2004 Elsevier Inc. All rights reserved
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4436
Polte, T., Tyrrell, R.M. (2004):
Involvement of lipid peroxidation and organic peroxides in UVA-induced matrix metalloproteinase-1 expression
Free Radic. Biol. Med. 36 (12), 1566 - 1574 10.1016/j.freeradbiomed.2004.04.003