Details zur Publikation
Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.biosystems.2004.06.003
Titel (primär) Analysis of two-component signal transduction by mathematical modeling using the KdpD/KdpE system of Escherichia coli
Autor Kremling, A.; Heermann, R.; Centler, F.; Jung, K.; Gilles, E.D.
Quelle Biosystems
Erscheinungsjahr 2004
Department UMB
Band/Volume 78
Heft 1-3
Seite von 23
Seite bis 37
Sprache englisch
Abstract A mathematical model for the KdpD/KdpE two-component system is presented and its dynamical behavior is analyzed. KdpD and KdpE regulate expression of the kdpFABC operon encoding the high affinity K+ uptake system KdpFABC of Escherichia coli. The model is validated in a two step procedure: (i) the elements of the signal transduction part are reconstructed in vitro. Experiments with the purified sensor kinase and response regulator in presence or absence of DNA fragments comprising the response regulator binding-site are performed. (ii) The mRNA and molecule number of KdpFABC are determined in vivo at various extracellular K+ concentrations. Based on the identified parameters for the in vitro system it is shown, that different time hierarchies appear which are used for model reduction. Then the model is transformed in such a way that a singular perturbation problem is formulated. The analysis of the in vivo system shows that the model can be separated into two parts (submodels which are called functional units) that are connected only in a unidirectional way. Hereby one submodel represents signal transduction while the second submodel describes the gene expression.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4271
Kremling, A., Heermann, R., Centler, F., Jung, K., Gilles, E.D. (2004):
Analysis of two-component signal transduction by mathematical modeling using the KdpD/KdpE system of Escherichia coli
Biosystems 78 (1-3), 23 - 37 10.1016/j.biosystems.2004.06.003