Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1002/pmic/200800836
Titel (primär) Proteome changes in human bronchoalveolar cells following styrene exposure indicate involvement of oxidative stress in the molecular-response mechanism
Autor Mörbt, N.; Mögel, I.; Kalkhof, S.; Feltens, R.; Röder-Stolinski, C.; Zheng, J.; Vogt, C.; Lehmann, I.; von Bergen, M.
Quelle Proteomics
Erscheinungsjahr 2009
Department ISOBIO; IMMU; METABOX; PROTEOM
Band/Volume 9
Heft 21
Seite von 4909
Seite bis 4933
Sprache englisch
Keywords Cell biology; Indoor air; Oxidative stress; Styrene; Styrene oxide-protein adducts; Volatile organic compound
Abstract Styrene is a volatile organic compound that is widely used as an intermediate in many industrial settings. There are known adverse health effects at environmentally significant concentrations, but little is known about the molecular effect of exposure to styrene at sub-acute toxic concentrations. We exposed human lung epithelial cells, at a wide range of concentrations (1 mg/m3-10 g/m3), to styrene and analyzed the effects on the proteome level by 2-DE, where 1380 proteins spots were detected and 266 were identified unambiguously by MS. A set of 16 protein spots were found to be significantly altered due to exposure to styrene at environmentally significant concentrations of 1-10 mg/m3 (0.2-2.3 ppm). Among these, superoxide dismutase as well as biliverdin reductase A could be correlated with the molecular pathway of oxidative stress, while eukaryotic translation initiation factor 5A-1, ezrin, lamin B2 and voltage-dependent anion channel 2 have been reported to be involved in apoptosis. Treatment with styrene also caused the formation of styrene oxide-protein adducts, specifically for thioredoxin reductase 1. These results underline the relevance of oxidative stress as a primary molecular response mechanism of lung epithelial cells to styrene exposure at indoor-relevant concentrations.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=423
Mörbt, N., Mögel, I., Kalkhof, S., Feltens, R., Röder-Stolinski, C., Zheng, J., Vogt, C., Lehmann, I., von Bergen, M. (2009):
Proteome changes in human bronchoalveolar cells following styrene exposure indicate involvement of oxidative stress in the molecular-response mechanism
Proteomics 9 (21), 4909 - 4933 10.1002/pmic/200800836