Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s11010-005-0169-x
Volltext Shareable Link
Titel (primär) Molecular cloning and expression of novel alternatively spliced cytochrome P450 2E1 mRNAs in humans
Autor Bauer, M.; Herbarth, O.; Aust, G.; Graebsch, C.
Quelle Molecular and Cellular Biochemistry
Erscheinungsjahr 2005
Department STUDIEN; EXPOEPID
Band/Volume 280
Heft 1-2
Seite von 201
Seite bis 207
Sprache englisch
Abstract Human cytochrome P450 2E1 (CYP2E1) is a phase I metabolizing enzyme. It is involved in the biotransformation of xenobiotics and endogenous substrates. Inter-individual genetic polymorphisms of the CYP2E1 gene are associated with different cancer diseases as well as alcohol and nicotine dependence. We report here for the first time three novel alternative spliced mRNA transcripts which are more frequently present in lung carcinoma cell lines as in hepatocyte cell lines. They are unexpected detectable in blood leukocytes from healthy volunteers but not in normal and cancerous lung tissue. The full-length wildtype transcript of CYP2E1 is described to be concomitant to an alternatively spliced mRNA transcript. Stimulation with CYP2E1-inducing agents did not change the splicing transcript pattern. The three splicing variants should lead to truncated non-functional proteins. Thus the genetic diversity of CYP2E1 is additionally extended at the transcriptional level of gene expression. The physiological role of the splicing variants is not known, yet, but they seem to be related to the carcinogenic property of the cell lines.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3166
Bauer, M., Herbarth, O., Aust, G., Graebsch, C. (2005):
Molecular cloning and expression of novel alternatively spliced cytochrome P450 2E1 mRNAs in humans
Mol. Cell. Biochem. 280 (1-2), 201 - 207 10.1007/s11010-005-0169-x