Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1099/mic.0.28875-0
Titel (primär) Contribution of alginate and levan production to biofilm formation by Pseudomonas syringae
Autor Laue, H.; Schenk, A.; Li, H.; Lambertsen, L.; Neu, T.R.; Molin, S.; Ullrich, M.S.
Quelle Microbiology-SGM
Erscheinungsjahr 2006
Department FLOEK
Band/Volume 152
Heft 10
Seite von 2909
Seite bis 2918
Sprache englisch
Abstract Exopolysaccharides (EPSs) play important roles in the attachment of bacterial cells to a surface and/or in building and maintaining the three-dimensional, complex structure of bacterial biofilms. To elucidate the spatial distribution and function of the EPSs levan and alginate during biofilm formation, biofilms of Pseudomonas syringae strains with different EPS patterns were compared. The mucoid strain PG4180.muc, which produces levan and alginate, and its levan- and/or alginate-deficient derivatives all formed biofilms in the wells of microtitre plates and in flow chambers. Confocal laser scanning microscopy with fluorescently labelled lectins was applied to investigate the spatial distribution of levan and an additional as yet unknown EPS in flow-chamber biofilms. Concanavalin A (ConA) bound specifically to levan and accumulated in cell-depleted voids in the centres of microcolonies and in blebs. No binding of ConA was observed in biofilms of the levan-deficient mutants or in wild-type biofilms grown in the absence of sucrose as confirmed by an enzyme-linked lectin-sorbent assay using peroxidase-linked ConA. Time-course studies revealed that expression of the levan-forming enzyme, levansucrase, occurred mainly during early exponential growth of both planktonic and sessile cells. Thus, accumulation of levan in biofilm voids hints to a function as a nutrient storage source for later stages of biofilm development. The presence of a third EPS besides levan and alginate was indicated by binding of the lectin from Naja mossambica to a fibrous structure in biofilms of all P. syringae derivatives. Production of the as yet uncharacterized additional EPS might be more important for biofilm formation than the syntheses of levan and alginate.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2815
Laue, H., Schenk, A., Li, H., Lambertsen, L., Neu, T.R., Molin, S., Ullrich, M.S. (2006):
Contribution of alginate and levan production to biofilm formation by Pseudomonas syringae
Microbiology-(UK) 152 (10), 2909 - 2918 10.1099/mic.0.28875-0