Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1093/jxb/eraa262
Titel (primär) Compatibility of X-ray computed tomography with plant gene expression profiling, rhizosphere bacterial community composition and enzyme activity analyses
Autor Ganther, M.; Yim, B.; Ibrahim, Z.; Bienert, M.D.; Lippold, E.; Maccario, L.; Sørensen, S.J.; Bienert, G.P.; Vetterlein, D.; Heintz-Buschart, A.; Blagodatskaya, E.; Smalla, K.; Tarkka, M.T.
Quelle Journal of Experimental Botany
Erscheinungsjahr 2020
Department BOOEK; iDiv; BOSYS
Band/Volume 71
Heft 18
Seite von 5603
Seite bis 5614
Sprache englisch
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Keywords bacterial community composition, extracellular soil enzyme, maize, plant elemental composition, rhizosphere, root gene expression, X-ray computed tomography
Abstract Non-invasive X-ray computed tomography (XRCT) is increasingly used in rhizosphere research to visualize development of soil–root interfaces in situ. However, exposing living systems to X-rays can potentially impact their processes and metabolites. In order to evaluate these effects, we assessed the responses of rhizosphere processes 1 h and 24 h after a low X-ray exposure (0.81 Gy). Changes in root gene expression patterns occurred 1 h after exposure with down-regulation of cell wall-, lipid metabolism-, and cell stress-related genes, but no differences remained after 24 h. At either time point, XRCT did not affect either root antioxidative enzyme activities, or the composition of rhizosphere bacterial microbiome and microbial growth parameters. The potential activities of leucine aminopeptidase and phosphomonoesterase were lower at 1 h, but did not differ from the control 24 h after exposure. A time delay of 24 h after a low X-ray exposure (0.81 Gy) was sufficient to revert any effects on the observed rhizosphere systems. Our data suggest that before implementing novel experimental designs involving XRCT, a study on its impacts on the investigated processes should be conducted.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23237
Ganther, M., Yim, B., Ibrahim, Z., Bienert, M.D., Lippold, E., Maccario, L., Sørensen, S.J., Bienert, G.P., Vetterlein, D., Heintz-Buschart, A., Blagodatskaya, E., Smalla, K., Tarkka, M.T. (2020):
Compatibility of X-ray computed tomography with plant gene expression profiling, rhizosphere bacterial community composition and enzyme activity analyses
J. Exp. Bot. 71 (18), 5603 - 5614 10.1093/jxb/eraa262