Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.copbio.2016.03.018
Titel (primär) Isotopes in geobiochemistry: tracing metabolic pathways in microorganisms of environmental relevance with stable isotopes
Autor Adrian, L.; Marco-Urrea, E.
Quelle Current Opinion in Biotechnology
Erscheinungsjahr 2016
Department ISOBIO
Band/Volume 41
Seite von 19
Seite bis 25
Sprache englisch
UFZ Querschnittsthemen RU3;
Abstract Stable isotopes are flexibly used as tracers to investigate environmental processes, microorganisms responsible for environmental transformations, syntrophic relationships in consortia, and metabolic pathways. With the advent of widely accessible high-resolution, highly accurate and sensitive mass spectrometers connected to liquid chromatography (LC–MS/MS) and the explosion of microbial genome sequence information the options to apply stable isotope tracers to geobiochemical topics have multiplied. With methods at hand to analyze biochemical pathways and enzymatic functions of yet-uncultivated microorganisms even in mixed cultures, a wide field of new discoveries can be expected. Applications rely both on the high sensitivity to detect trace amounts of biological material in slow or non-growing cultures and on the high multi-dimensional resolution of LC–MS/MS to allow the separation of complex samples and to retrieve phylogenetic information. Challenges and examples of stable isotope applications to describe geobiochemical processes are reviewed. Overall, the potential is not yet sufficiently deployed.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17523
Adrian, L., Marco-Urrea, E. (2016):
Isotopes in geobiochemistry: tracing metabolic pathways in microorganisms of environmental relevance with stable isotopes
Curr. Opin. Biotechnol. 41 , 19 - 25 10.1016/j.copbio.2016.03.018