Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.sab.2015.12.001
Titel (primär) Determination of trace elements in freshwater rotifers and ciliates by total reflection X-ray fluorescence spectrometry
Autor Woelfl, S.; Óvári, M.; Nimptsch, J.; Neu, T.R.; Mages, M.
Quelle Spectrochimica Acta Part B: Atomic Spectroscopy
Erscheinungsjahr 2016
Department FLOEK
Band/Volume 116
Seite von 28
Seite bis 33
Sprache englisch
Keywords Trace element analysis; Total reflection X-ray fluorescence; Rotatoria; Ciliates; Bioaccumulation
UFZ Querschnittsthemen RU2;
Abstract Element determination in plankton is important for the assessment of metal contamination of aquatic environments. Until recently, it has been difficult to determine elemental content in rotifers or ciliates derived from natural plankton samples because of the difficulty in handling and separation of these fragile organisms. The aim of this study was to evaluate methods for separation of rotifers and large ciliates from natural plankton samples (μg range dry weight) and subsequent analysis of their elemental content using total-reflection X-ray fluorescence spectrometry (TXRF). Plankton samples were collected from different aquatic environments (three lakes, one river) in Chile, Argentina and Hungary. From one to eighty specimens of five rotifer species (Brachionus calyciflorus, Brachionus falcatus, Asplanchna sieboldii, Asplanchna sp., Philodina sp.) and four to twelve specimens of one large ciliate (Stentor amethystinus) were prepared according to the dry method originally developed for microcrustaceans, and analysed by TRXF following in situ microdigestion. Our results demonstrated that it possible to process these small and fragile organisms (individual dry mass: 0.17–9.39 μg ind- 1) via careful washing and preparation procedures. We found species-dependent differences of the element mass fractions for some of the elements studied (Cr, Mn, Fe, Ni, Cu, Zn, As, Pb), especially for Cu, Fe and Mn. One large rotifer species (A. sieboldii) also showed a negative correlation between individual dry weight and the element content for Pb, Ni and Cr. We conclude that our application of the in situ microdigestion-TRXF method is suitable even for rotifers and ciliates, greatly expanding the possibilities for use of plankton in biomonitoring of metal contamination in aquatic environments.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17001
Woelfl, S., Óvári, M., Nimptsch, J., Neu, T.R., Mages, M. (2016):
Determination of trace elements in freshwater rotifers and ciliates by total reflection X-ray fluorescence spectrometry
Spectroc. Acta Pt. B-Atom. Spectr. 116 , 28 - 33 10.1016/j.sab.2015.12.001