Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1186/s13148-015-0113-1
Titel (primär) A varying T cell subtype explains apparent tobacco smoking induced single CpG hypomethylation in whole blood
Autor Bauer, M.; Linsel, G.; Fink, B.; Offenberg, K.; Hahn, A.M.; Sack, U.; Knaack, H.; Eszlinger, M.; Herberth, G. ORCID logo
Quelle Clinical Epigenetics
Erscheinungsjahr 2015
Department IMMU
Band/Volume 7
Seite von art. 81
Sprache englisch
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Keywords DNA methylation; CpG; GPR15; Tobacco smoking; Blood; EWAS cell composition
UFZ Querschnittsthemen RU3;
Abstract

Background

Many recent epigenetic studies report that cigarette smoking reduces DNA methylation in whole blood at the single CpG site cg19859270 within the GPR15 gene.

Results

Within two independent cohorts, we confirmed the differentially expression of the GPR15 gene when smokers and non-smokers subjects are compared. By validating the GPR15 protein expression at the cellular level, we found that the observed decreased methylation at this site in white blood cells (WBC) of smokers is mainly caused by the high proportion of CD3+GPR15+ expressing T cells in peripheral blood. In current smokers, the percentage of GPR15+ cells among CD3+ T cells in peripheral blood is significantly higher (15.5 ± 7.2 %, mean ± standard deviation) compared to non-smokers (3.7 ± 1.6 %). Treatment of peripheral blood mononuclear cell (PBMC) cultures with aqueous cigarette smoke extract did not induce a higher proportion of this T cell subtype.

Conclusions

Our results underline that DNA hypomethylation at cg19859270 site, observed in WBCs of smokers, did not arise by direct effect of tobacco smoking compounds on methylation of DNA but rather by the enrichment of a tobacco-smoking-induced lymphocyte population in the peripheral blood.

dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16393
Bauer, M., Linsel, G., Fink, B., Offenberg, K., Hahn, A.M., Sack, U., Knaack, H., Eszlinger, M., Herberth, G. (2015):
A varying T cell subtype explains apparent tobacco smoking induced single CpG hypomethylation in whole blood
Clin. Epigenetics 7 , art. 81 10.1186/s13148-015-0113-1