Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s10532-014-9684-3
Volltext Shareable Link
Titel (primär) Adaptation of Delftia acidovorans for degradation of 2,4-dichlorophenoxyacetate in a microfluidic porous medium
Autor Yoon, H.; Leibeling, S.; Zhang, C.; Müller, R.H.; Werth, C.J.; Zilles, J.L.
Quelle Biodegradation
Erscheinungsjahr 2014
Department UMB
Band/Volume 25
Heft 4
Seite von 595
Seite bis 604
Sprache englisch
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs10532-014-9684-3/MediaObjects/10532_2014_9684_MOESM1_ESM.pdf
UFZ Querschnittsthemen RU3;
Abstract Delftia acidovorans MC1071 can productively degrade R-2-(2,4-dichlorophenoxy)propionate (R-2,4-DP) but not 2,4-dichlorophenoxyacetate (2,4-D) herbicides. This work demonstrates adaptation of MC1071 to degrade 2,4-D in a model two-dimensional porous medium (referred to here as a micromodel). Adaptation for 2,4-D degradation in the 2 cm-long micromodel occurred within 35 days of exposure to 2,4-D, as documented by substrate removal. The amount of 2,4-D degradation in the adapted cultures in two replicate micromodels (~10 and 20 % over 142 days) was higher than a theoretical maximum (4 %) predicted using published numerical simulation methods, assuming instantaneous biodegradation and a transverse dispersion coefficient obtained for the same pore structure without biomass present. This suggests that the presence of biomass enhances substrate mixing. Additional evidence for adaptation was provided by operation without R-2,4-DP, where degradation of 2,4-D slowly decreased over 20 days, but was restored almost immediately when R-2,4-DP was again provided. Compared to suspended growth systems, the micromodel system retained the ability to degrade 2,4-D longer in the absence of R-2,4-DP, suggesting slower responses and greater resilience to fluctuations in substrates might be expected in the soil environment than in a chemostat.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=15013
Yoon, H., Leibeling, S., Zhang, C., Müller, R.H., Werth, C.J., Zilles, J.L. (2014):
Adaptation of Delftia acidovorans for degradation of 2,4-dichlorophenoxyacetate in a microfluidic porous medium
Biodegradation 25 (4), 595 - 604 10.1007/s10532-014-9684-3